High potassium-induced activation of choline-acetyltransferase in human neocortex: implications and species differences

Brain Research Bulletin
J-P SigleT J Feuerstein

Abstract

The role of electrical and potassium (K(+))-induced depolarisation on choline-acetyltransferase (ChAT) activity in human and mouse neocortical slices was studied. When [3H]-ACh release was evoked by two K(+) stimulations in human neocortex, the mean S(2)/S(1) ratio was significantly below unity. ChAT inhibitors, like bromo-acetylcholine and ocadaic acid, raised this ratio by 79 and 63%, respectively, suggesting that the diminished S(2)/S(1) value in the absence of ChAT inhibitors reflected an increased ChAT activity at S(2) following K(+) depolarisation at S(1). When stimulated electrically, however, the S(2)/S(1) ratio in human neocortex was near unity and ocadaic acid remained without effect. In parallel experiments on mouse neocortical slices, the S(2)/S(1) ratio was near unity in both electrically or K(+)-evoked [3H]-ACh release and was not altered by ChAT inhibition. ChAT activity following K(+) depolarisation was also determined directly. ChAT activation in human neocortical slices was highest at 10 and 20mM K(+). ChAT activity in mouse neocortical tissue was not altered by K(+) depolarisation. These results suggest that in human, but not in mouse, neocortex ChAT activity may be increased due to ongoing K(+) depolarisatio...Continue Reading

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Citations

Jun 1, 2010·Autonomic Neuroscience : Basic & Clinical·Obaida R RanaErol Saygili
Nov 29, 2007·The Journal of Comparative Neurology·Mary Ann RaghantiChet C Sherwood
Jan 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jung-Hee JangChangjong Moon

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