High precision and fast functional mapping of cortical circuitry through a novel combination of voltage sensitive dye imaging and laser scanning photostimulation.

Journal of Neurophysiology
Xiangmin XuZoran Nenadic

Abstract

The development of modern neuroscience tools is critical for deciphering brain circuit organization and function. An important aspect for technical development is to enhance each technique's advantages and compensate for limitations. We developed a high-precision and fast functional mapping technique in brain slices that incorporates the spatial precision of activation that can be achieved by laser-scanning photostimulation with rapid and high-temporal resolution assessment of evoked network activity that can be achieved by voltage-sensitive dye imaging. Unlike combination of whole cell recordings with photostimulation for mapping local circuit inputs to individually recorded neurons, this innovation is a new photostimulation-based technique to map cortical circuit output and functional connections at the level of neuronal populations. Here we report on this novel technique in detail and show its effective applications in mapping functional connections and circuit dynamics in mouse primary visual cortex and hippocampus. Given that this innovation enables rapid mapping and precise evaluation of cortical organization and function, it can have broad impacts in the field of cortical circuitry.

References

Oct 15, 1978·The Journal of Comparative Neurology·L W SwansonW M Cowan
Jan 8, 1989·The Journal of Comparative Neurology·A Burkhalter
Jan 1, 1983·Annual Review of Neuroscience·C D Gilbert
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E M Callaway, L C Katz
Apr 8, 1998·Annual Review of Neuroscience·E M Callaway
Jun 22, 2000·Nature Neuroscience·J L Dantzker, E M Callaway
Mar 19, 2002·Journal of Neuroscience Methods·Wenjun JinJian-young Wu
Oct 22, 2004·Nature Reviews. Neuroscience·Amiram Grinvald, Rina Hildesheim
Nov 5, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaoying HuangJian-Young Wu
Jun 17, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gordon M G Shepherd, Karel Svoboda
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Nov 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chyze W AngDouglas A Coulter
Oct 30, 2007·Nature Methods·Volodymyr NikolenkoRafael Yuste
Feb 5, 2008·Nature Neuroscience·Nicholas WeilerGordon M G Shepherd
Mar 18, 2008·Neuron·Liqun LuoKarel Svoboda
Jan 9, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiangmin Xu, Edward M Callaway

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Citations

Aug 27, 2013·Nature·Sandra J KuhlmanJoshua T Trachtenberg
Oct 19, 2011·Journal of Visualized Experiments : JoVE·Taruna IkrarXiangmin Xu
Mar 19, 2014·The Journal of Physiology·Gregory S ParksXiangmin Xu
Nov 9, 2017·PloS One·Stefan PasslickGraham C R Ellis-Davies
Mar 1, 2019·Journal of Neural Engineering·Yuta TanakaTetsuya Yagi
Jun 27, 2020·Frontiers in Neural Circuits·Cristian MoralesNoelia V Weisstaub

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