High-pressure cryoimmobilization of murine skin reveals novel structural features and prevents extraction artifacts

Experimental Dermatology
S ReipertG Wiche

Abstract

Cryoimmobilization by high-pressure freezing (HPF) and subsequent freeze substitution has been proven as an effective method to preserve tissues. Here, we demonstrate for the first time that a comprehensive morphological and ultrastructural preservation of mouse skin throughout all its layers can be achieved in this way. Using conditions limiting tissue-extraction during freeze substitution, we could prevent the massive interdigitation of cell membranes, the loss of tubular structures of the Golgi complex, the aggregation of keratin to electron-dense bundles, the formation of round-shaped keratohyalin aggregates, the dispersion of locally organized ribosomes, the excessive aggregation of material at hemidesmosomal plaques, the massive extraction of material from the basement membrane and the adjacent dermal region, and the dissociation of components of the dermal matrix. Taken together, HPF in combination with freeze substitution emerges as a highly sensitive tool for morphological and ultrastructural analysis.

References

Jan 1, 1986·The Journal of Investigative Dermatology·M J Tidman, R A Eady
Aug 1, 1994·Journal of Cutaneous Pathology·G Schaumburg-LeverP Nau
May 1, 1994·Microscopy Research and Technique·F L Chan, S Inoue
Mar 1, 1994·The Journal of Investigative Dermatology·N AokiH Ozawa
Aug 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D R Keene, K McDonald
Feb 1, 1993·Current Opinion in Cell Biology·D R Garrod
Aug 1, 1996·Journal of Microscopy·H HohenbergM Müller
Feb 1, 1996·Archives of Dermatological Research·J R McMillan, R A Eady
Aug 15, 1997·Microscopy Research and Technique·D R KeeneL Y Sakai
Sep 12, 1998·The Journal of Investigative Dermatology. Symposium Proceedings·P M EliasG K Menon
Jan 29, 1999·Journal of Microscopy·P MonaghanM Müller
Apr 14, 1999·The Journal of Investigative Dermatology·L Borradori, A Sonnenberg
Jun 24, 1999·Journal of Dermatological Science·D Zillikens
Sep 14, 2001·Journal of Microscopy·D StuderP Eggli
Sep 17, 2002·The Journal of Investigative Dermatology·Ushio TakedaHiroshi Shinkai
Mar 22, 2003·The Journal of Investigative Dermatology·Lars NorlénJaques Dubochet

❮ Previous
Next ❯

Citations

Sep 18, 2007·Anatomical Science International·Sachiko MatsuuraAkio Kikuta
May 29, 2015·Cell and Tissue Research·Gernot WalkoGerhard Wiche
Dec 10, 2014·Cell and Tissue Research·Gernot WalkoGerhard Wiche
Jun 30, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Douglas R Keene, Sara F Tufa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.