High-pressure stabilization of alpha-chymotrypsin entrapped in reversed micelles of aerosol OT in octane against thermal inactivation

FEBS Letters
R V RariyC Balny

Abstract

alpha-Chymotrypsin (CT) solubilized in reversed micelles of sodium bis-(2-ethylhexyl)-sulfosuccinate (AOT) undergoes thermal inactivation and the enzyme stability decreases significantly when temperature increases (25-40 degrees C). The half-life of CT in micelles shows a bell-shaped dependence on the degree of hydration of AOT (wo) analogous to the previously obtained dependence on wo for the enzyme activity. The optima of catalytic activity and thermal stability have been observed under conditions where the diameter of the inner aqueous cavity of the micelle is close to the size of the enzyme molecule (wo = 10). Application of high hydrostatic pressure in the range of 1-1500 atm (bar) stabilizes CT against thermal inactivation at all hydration degrees (wo) from 7 to 20; the stabilization effect is most pronounced under the experimental conditions being far from the optimum for catalytic activity.

References

Mar 31, 1995·Annals of the New York Academy of Sciences·V V MozhaevC Balny
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·J Jonas, A Jonas
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Feb 20, 1994·Biotechnology and Bioengineering·R AffleckA J Russell

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Citations

Oct 20, 1996·Biotechnology and Bioengineering·V V MozhaevC Balny
Nov 15, 1995·Biochimica Et Biophysica Acta·C CleryP Masson
Jun 6, 2003·Chemistry and Physics of Lipids·Eduardo P MeloSteffen B Petersen
May 2, 2002·Biochimica Et Biophysica Acta·László Smeller
May 28, 2009·Journal of the American Chemical Society·Wade D Van HornPeter F Flynn
Mar 31, 1995·Annals of the New York Academy of Sciences·A V Levashov, N Klyachko
Sep 24, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Trung Quan LuongRoland Winter
Mar 28, 2018·Annual Review of Food Science and Technology·José I Reyes-De-CorcueraRosalía García-Torres
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Filip MeersmanKarel Heremans
Jan 28, 1999·Biochemical and Biophysical Research Communications·N L KlyachkoC Balny
Jul 27, 2006·Journal of the American Chemical Society·Alana K Simorellis, Peter F Flynn
Apr 13, 2006·Journal of the American Chemical Society·Alana K SimorellisPeter F Flynn
Sep 30, 2005·Journal of the American Chemical Society·Wade D Van HornPeter F Flynn

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