High prevalence and characterization of extended-spectrum ß-lactamase producing Enterobacteriaceae in Chadian hospitals

BMC Infectious Diseases
Oumar Ouchar MahamatSylvain Godreuil

Abstract

Extended-spectrum ß-lactamase-producing Enterobacteriaceae (ESBL-PE) represent a major problem in the management of nosocomial infections. However, ESBL-PE are not systematically monitored in African countries. The aim of this study was to determine ESBL-PE prevalence in patients from three hospitals in N'Djamena, the capital city of Chad, and to characterize the genetic origin of the observed resistance. From January to March 2017, 313 non-duplicate isolates were recovered from various clinical specimens obtained from 1713 patients in the three main hospitals of N'Djamena. Bacterial species were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Susceptibility to 28 antibiotics was tested using the disk diffusion method on Müller-Hinton agar, and ESBL production was confirmed with the double-disc synergy test. The most prevalent ESBL genes associated with the observed resistance were detected using multiplex PCR followed by double-stranded DNA sequencing. Among the 313 isolates, 197 belonged to the Enterobacteriaceae family. The overall ESBL-PE prevalence was 47.72% (n = 94/197), with a higher rate among inpatients compared with outpatients (54.13% vs. 34.37%). ESBL-PE prevalence was hi...Continue Reading

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Citations

May 10, 2020·Surgical Infections·Jennifer Rickard
Nov 7, 2019·Antimicrobial Resistance and Infection Control·Oumar Ouchar MahamatSylvain Godreuil
Jan 16, 2020·BMC Infectious Diseases·Luis Furuya-Kanamori, Laith Yakob
May 7, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Annicet-Clotaire DikoumbaSylvain Godreuil
May 8, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Sophia V KazakovaL Clifford McDonald
Jun 15, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Onduru G OnduruSusan F Rumisha

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Methods Mentioned

BETA
PCR
PCRs
electrophoresis

Software Mentioned

BLAST

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