High prevalence of dihydrofolate reductase gene mutations in Plasmodium falciparum parasites among pregnant women in Nigeria after reported use of sulfadoxine-pyrimethamine

Pathogens and Global Health
Olusola OjurongbeThirumalaisamy P Velavan

Abstract

This study assesses the prevalence of asymptomatic Plasmodium falciparum parasitemia positivity and P. falciparum dihydrofolate reductase (pfdhfr) mutations in parasite isolates among pregnant women in Southwest Nigeria. Plasmodium falciparum parasitemia was confirmed by microscopy and nested PCR in 200 pregnant women attending antenatal care. The prevalence of pfdhfr polymorphisms was determined by direct sequencing of the gene fragments containing the C50R, N51I, C59R, S108N, and I164L mutations. Information on the use of antimalarial drugs and methods applied to prevent malaria were obtained by a questionnaire. The prevalence of asymptomatic P. falciparum infection was 30% (60/200). The frequency of the pfdhfr triple-mutant alleles (N51I, C59R, and S108N) was 63% (38/60); none of the isolates carried the I164L mutation. Among the investigated pregnant women, 40% used un-prescribed antimalarials such as dihydroartemisinin (18%), chloroquine (14%) or pyrimethamine (9%), while only 20.5% used sulfadoxine-pyrimethamine for prevention and 39.5% did not use any drug. The prevalence of P. falciparum parasitemia (37%) was higher among pregnant women who had not taken any antimalarial drugs. A significant difference in the prevalence...Continue Reading

References

Jan 22, 1955·British Medical Journal·R N CHAUDHURI, N K CHAKRAVARTY
Nov 19, 2003·Current Opinion in Infectious Diseases·Walther Helmut Wernsdorfer, Harald Noedl
May 25, 2004·Antimicrobial Agents and Chemotherapy·Michelle L GattonQin Cheng
Jan 26, 2007·The Lancet Infectious Diseases·Meghna DesaiRobert D Newman
Nov 14, 2007·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·M B KaguG B Gadzama
May 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·W E HarringtonP E Duffy
Jun 3, 2009·The Korean Journal of Parasitology·Chimere O AgomoPhilip U Agomo
Jun 16, 2009·Biologicals : Journal of the International Association of Biological Standardization· Who
Feb 4, 2010·PLoS Medicine·Stephanie DellicourFeiko O ter Kuile
Feb 6, 2010·The American Journal of Tropical Medicine and Hygiene·Marielle K Bouyou-AkotetMaryvonne Kombila
Dec 2, 2010·Wiener klinische Wochenschrift·Olusola OjurongbeJürgen F Kun
Apr 20, 2011·Studies in Family Planning·UNKNOWN National Population Commission, UNKNOWN ICF Macro
Jul 8, 2011·The American Journal of Tropical Medicine and Hygiene·Nana O WilsonJonathan K Stiles
Jul 19, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Whitney E HarringtonPatrick E Duffy
Aug 20, 2011·BMC Pregnancy and Childbirth·Olukemi O TongoOlusegun O Akinyinka
Aug 24, 2013·Emerging Infectious Diseases·Daniel T R MinjaMichael Alifrangis
Sep 18, 2013·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Nkechi G OnyenehoWilliam R Brieger
Nov 9, 2016·International Journal for Parasitology. Drugs and Drug Resistance·Mary C OguikeCally Roper

❮ Previous
Next ❯

Citations

Oct 6, 2018·Expert Opinion on Pharmacotherapy·Brioni R Moore, Timothy M E Davis
Jul 31, 2020·Expert Opinion on Drug Metabolism & Toxicology·Brioni R Moore, Timothy M Davis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.