High prevalence of Helicobacter pylori clarithromycin resistance mutations among Seattle patients measured by droplet digital PCR

Helicobacter
Sarah TalaricoNina R Salama

Abstract

Treatment of Helicobacter pylori infection is often empiric; however, current guidelines for management of Helicobacter pylori infection advise against the use of standard triple therapy (clarithromycin, amoxicillin, and proton-pump inhibitor) when clarithromycin resistance exceeds 20%. We developed and tested a new culture-free assay to detect clarithromycin resistance-conferring mutations to determine the prevalence of H. pylori clarithromycin resistance in patients from the United States Pacific Northwest. Droplet digital PCR (ddPCR) was used to detect the H. pylori 23S rRNA gene, and resistance-conferring mutations, in archived, formalin-fixed, paraffin-embedded (FFPE) gastric tissue and to retrospectively determine the prevalence of clarithromycin-resistant H. pylori among 110 patients at an academic medical center in the Northwest United States between 2012 and 2014. Of 102 patients with the H. pylori 23S rRNA gene detected by the ddPCR assay, 45 (44%) had clarithromycin resistance mutations. Thirty-three of the 45 patients with clarithromycin resistance mutations had a mix of wild-type and resistance alleles. Prevalence of clarithromycin resistance mutations differed among racial groups and was highest among Asians, with...Continue Reading

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Citations

Nov 30, 2018·Current Opinion in Gastroenterology·Sinéad M SmithDeirdre McNamara
Aug 23, 2019·Current Opinion in Gastroenterology·Erick A Argueta, Steven F Moss
May 17, 2019·The American Journal of Gastroenterology·Doron BoltinShlomo Birkenfeld
Sep 6, 2019·Helicobacter·Athanasios MakristathisEmilie Bessède
Jun 23, 2020·Clinical and Translational Gastroenterology·María José Ramírez-LázaroXavier Calvet
Jul 10, 2019·Southern Medical Journal·Benjamin R Lloyd, David A Leiman
May 19, 2021·Nature Reviews. Gastroenterology & Hepatology·Evariste Tshibangu-Kabamba, Yoshio Yamaoka
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes
Aug 28, 2021·Diagnostics·Hang Yang, Bing Hu

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