Abstract
The spotted fever group (SFG) rickettsiae are obligate intracellular bacteria transmitted by ticks that cause several tick-borne rickettsioses in humans worldwide. This study was intended to determine the prevalence of SFG rickettsiae in Amblyomma variegatum from 7 districts across Uganda. In addition to sequencing of gltA and ompA genes, identification of Rickettsia species based on the sizes of highly variable intergenic spacers, namely, dksA-xerC, mppA-purC, and rpmE-tRNA(fMet) was carried out. Application of multiplex PCR for simultaneous amplification of 3 spacers combined with capillary electrophoresis separation allowed simple, accurate, and high-throughput fragment sizing with considerable time and cost savings. Rickettsia genus-specific real-time PCR detected 136 positives out of 140 samples, giving an overall prevalence of 97.1%. Most samples (n=113) had a size combination of 225, 195, and 341 bp for dksA-xerC, mppA-purC, and rpmE-tRNA(fMet), respectively, which was identical to that of R. africae, a causative agent of African tick bite fever. In addition, several samples had size variants in either dksA-xerC or rpmE-tRNA(fMet). Nonetheless, the partial sequences of gltA and ompA genes of samples of all size combinati...Continue Reading
References
Dec 19, 2001·Emerging Infectious Diseases·P ParolaD Raoult
Sep 5, 2003·The Lancet Infectious Diseases·Mogens JenseniusDidier Raoult
Nov 26, 2003·Microbiology and Immunology·Mitsuhiro IshikuraMamoru Watanabe
Dec 10, 2003·Journal of Clinical Microbiology·Pierre-Edouard FournierDidier Raoult
Apr 6, 2004·International Journal for Parasitology·C A L OuraA Tait
Dec 8, 2004·Journal of Clinical Microbiology·Pierre-Edouard FournierDidier Raoult
Oct 15, 2005·Clinical Microbiology Reviews·Philippe ParolaDidier Raoult
Jul 31, 2007·BMC Microbiology·Pierre-Edouard Fournier, Didier Raoult
Feb 9, 2008·Emerging Infectious Diseases·Cristina SocolovschiPhilippe Parola
Aug 30, 2008·Infectious Disease Clinics of North America·Cécile CazorlaPhilippe Parola
Mar 31, 2009·Applied and Environmental Microbiology·Felicitas S BorettiRegina Hofmann-Lehmann
May 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C SocolovschiP Parola
Oct 29, 2009·Emerging Infectious Diseases·Leonard IzzardJohn Stenos
Nov 7, 2009·Emerging Infectious Diseases·Mogens JenseniusUNKNOWN GeoSentinel Surveillance Network
Jan 7, 2010·PloS One·Karin ElfvingKenneth Nilsson
Mar 6, 2010·Emerging Infectious Diseases·Oleg MediannikovDidier Raoult
Aug 14, 2010·Medical and Veterinary Entomology·G Dharmarajan, O E Rhodes
Aug 26, 2010·Emerging Infectious Diseases·Cristina SocolovschiDidier Raoult
Nov 20, 2010·BMC Microbiology·Ryo NakaoChihiro Sugimoto
Jan 5, 2011·Emerging Infectious Diseases·Carole EldinPhilippe Parola
Feb 5, 2011·Emerging Infectious Diseases·Leonid RumerMatthias Niedrig
Apr 19, 2011·Experimental & Applied Acarology·J W MagonaM C Eisler
Jul 19, 2011·Parasites & Vectors·Ryo NakaoChihiro Sugimoto
Jul 27, 2011·Current Opinion in Infectious Diseases·Eyal LeshemEli Schwartz
Oct 20, 2011·Parasitology·Ryo NakaoChihiro Sugimoto
Nov 10, 2011·FEMS Immunology and Medical Microbiology·Allen L Richards
Jan 10, 2012·Vector Borne and Zoonotic Diseases·Chang-wei LiangXue-jie Yu
Feb 7, 2012·Emerging Infectious Diseases·Alice N MainaM Kariuki Njenga
Feb 9, 2012·Ticks and Tick-borne Diseases·Oleg MediannikovDidier Raoult
Sep 13, 2012·PloS One·Nathalie AcestorDavid Bell
Nov 9, 2012·Journal of Clinical Microbiology·Cecilia Y KatoRobert F Massung
Citations
Oct 18, 2014·Vector Borne and Zoonotic Diseases·Alice N MainaAllen L Richards
Jan 5, 2016·Ticks and Tick-borne Diseases·Julian EhlersSven Poppert
May 7, 2016·Ticks and Tick-borne Diseases·Paul Franck Adjou MoumouniXuenan Xuan
May 20, 2015·Vector Borne and Zoonotic Diseases·Ryo NakaoChihiro Sugimoto
Sep 21, 2016·Emerging Infectious Diseases·Petra BogovicTatjana Avsic-Zupanc
Sep 20, 2016·Ticks and Tick-borne Diseases·L TomassoneE Grego
Mar 14, 2013·Veterinary Parasitology·Amanda D LoftisChengming Wang
Jun 1, 2018·The American Journal of Tropical Medicine and Hygiene·Cara C CherryChristopher D Paddock
Feb 20, 2019·Vector Borne and Zoonotic Diseases·May June ThuRyo Nakao
May 28, 2019·Frontiers in Physiology·Larissa A MartinsAndréa C Fogaça
Sep 14, 2019·Microbiology Resource Announcements·May June ThuRyo Nakao
Nov 18, 2015·Parasitology Research·Yongjin QiuChihiro Sugimoto
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Dong-Sheng Lian, Shu-Jin Zhao
Feb 8, 2019·Scientific Reports·May June ThuRyo Nakao
Aug 5, 2020·Pathogens·Benson Chuks IwerieborChikwelu Larry Obi
Oct 8, 2020·Experimental & Applied Acarology·Alicia D Pillay, S Mukaratirwa
Nov 4, 2020·Transboundary and Emerging Diseases·Tatenda ChiuyaJandouwe Villinger
Apr 6, 2021·Ticks and Tick-borne Diseases·Min-Goo SeoDongmi Kwak
Jun 24, 2016·Microbiology Spectrum·Tahar KernifPhilippe Parola
Jul 3, 2021·Pathogens·Toshiya KobayashiRyo Nakao