High prevalence of triazole resistance in Aspergillus fumigatus, especially mediated by TR/L98H, in a French cohort of patients with cystic fibrosis

The Journal of Antimicrobial Chemotherapy
Florent MorioPatrice Le Pape

Abstract

Triazole resistance in Aspergillus fumigatus due to a single azole resistance mechanism (TR/L98H) is increasingly reported in European countries. Data from patients with cystic fibrosis (CF) are limited. Our study aimed to investigate the prevalence and molecular mechanisms of azole resistance in A. fumigatus in a cohort of patients with CF. Eighty-five A. fumigatus isolates from 50 CF patients, collected between January 2010 and April 2011, were retrospectively analysed for azole resistance using agar plates containing 4 mg/L itraconazole. MICs of itraconazole, voriconazole and posaconazole were determined according to EUCAST methodology for each isolate able to grow on this medium. Species identification was performed by sequencing of the β-tubulin gene. Sequencing analysis of the cyp51A gene and its promoter region was conducted. Nine isolates (four patients, 8% prevalence) were able to grow on itraconazole-containing agar plates. Itraconazole resistance was confirmed by EUCAST methodology (MICs >2 mg/L). All isolates had mutations in the cyp51A gene at residues previously involved in azole resistance: L98H (n = 5), M220T (n = 4) and G54R (n = 1). One patient had three genetically distinct azole-resistant isolates identified...Continue Reading

References

Jun 1, 1997·Antimicrobial Agents and Chemotherapy·D W DenningS L Kelly
Aug 15, 1998·Journal of Clinical Microbiology·E Bart-DelabesseS Bretagne
Mar 10, 2005·Eukaryotic Cell·S Arunmozhi BalajeeKieren A Marr
Oct 3, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·UNKNOWN Subcommittee on Antifungal Susceptibility Testing of the ESCMID European Committee for Antimicrobial Susceptibility Testing
Jul 25, 2009·Emerging Infectious Diseases·Susan J HowardDavid W Denning
Nov 3, 2009·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Paul E VerweijDavid W Denning
Nov 21, 2009·The Lancet Infectious Diseases·Paul E VerweijWillem J G Melchers
Sep 2, 2010·Antimicrobial Agents and Chemotherapy·Klaus Leth MortensenMaiken Cavling Arendrup
Dec 7, 2010·The Journal of Antimicrobial Chemotherapy·Alexandre AlanioStéphane Bretagne
Apr 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David W DenningDavid S Perlin
Oct 18, 2011·Emerging Infectious Diseases·Jan W M van der LindenPaul E Verweij
Oct 27, 2011·The Journal of Antimicrobial Chemotherapy·Anuradha ChowdharyJacques F Meis
Nov 30, 2011·Antimicrobial Agents and Chemotherapy·Pierre-Régis BurgelAndré Paugam

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Citations

May 7, 2013·Asian Pacific Journal of Tropical Biomedicine·Stephen ChegeVincent Obanda
Sep 12, 2012·Current Opinion in Pulmonary Medicine·Jennifer J SpeirsJeffrey M Beekman
Feb 16, 2013·Current Opinion in Pulmonary Medicine·Gemma E Hayes, David W Denning
May 1, 2013·Antimicrobial Agents and Chemotherapy·Pilar EscribanoJesús Guinea
Jul 31, 2012·Eukaryotic Cell·Sanjoy PaulW Scott Moye-Rowley
May 9, 2013·Medical Mycology·Lilian Masoud-LandgrafWalter Buzina
Aug 26, 2014·Emerging Infectious Diseases·Cau D PhamShawn R Lockhart
Feb 6, 2016·Future Microbiology·Leticia Bernal-MartínezManuel Cuenca-Estrella
Apr 9, 2013·Médecine et maladies infectieuses·L LelièvreM-E Bougnoux
Dec 4, 2015·Journal de mycologie médicale·S RocchiL Millon
Dec 13, 2012·Annals of the New York Academy of Sciences·Sarah P Georgiadou, Dimitrios P Kontoyiannis
May 13, 2014·Transplant Infectious Disease : an Official Journal of the Transplantation Society·F S de FontbruneA Alanio
Apr 26, 2013·Pest Management Science·Paul Bowyer, David W Denning
Sep 1, 2015·Evolution; International Journal of Organic Evolution·Jianhua ZhangSijmen E Schoustra
Jan 3, 2015·Fungal Genetics and Biology : FG & B·Fei-fei LiuLing Lu
Jan 1, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C Arendrup
Jun 30, 2015·Current Clinical Microbiology Reports·David S PerlinYanan Zhao
Mar 15, 2014·The International Journal of Biochemistry & Cell Biology·Sanjay H Chotirmall, Noel G McElvaney
Jul 1, 2016·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Anna PrigitanoAnna Maria Tortorano
Apr 21, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maurine D'AgostinoAnne Debourgogne
Apr 29, 2015·Antimicrobial Agents and Chemotherapy·Rose-Anne LavergnePatrice Le Pape
Apr 22, 2015·Antimicrobial Agents and Chemotherapy·Musang LiuWeida Liu
Aug 18, 2017·Journal of Clinical Microbiology·Eric DannaouiMarie-Elisabeth Bougnoux
May 20, 2015·Antimicrobial Agents and Chemotherapy·Edith VermeulenKatrien Lagrou
Oct 16, 2013·Current Opinion in Infectious Diseases·Edith VermeulenPaul E Verweij
Aug 14, 2019·International Journal of Environmental Health Research·Daniela SimõesRaquel Sabino
Jul 23, 2019·Medical Mycology·Beatriz BustamantePablo E Campos

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