High promoter activity of cytokeratin-19 gene in cholangiocarcinoma

Molecular and Clinical Oncology
Jian QinZhi-Hai Peng

Abstract

Conditionally replicating adenoviral vectors constructed with tumor-specific promoters (TSPs) offers a viable tool for the treatment of cholangiocarcinoma. The aim of the present study was to investigate cholangiocarcinoma-specific TSPs that remain active in adenoviral constructs in gene therapy. The mRNA expressions of cyclooxygenase-2, cytokeratin-19 (CK19), mucin-1, midkine and telomerase reverse transcriptase were determined in human cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using reverse transcription-quantitative polymerase chain reaction. The candidate promoters constructed in adenoviral vectors were analyzed for their activities in cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using dual-luciferase reporter assays. The mRNA expression of CK19 was markedly higher in the QBC939 cell line, indicating specificity to cholangiocarcinoma. Moreover, the promoter activity of CK19 in the adenoviral vector in infected cholangiocarcinoma cells was found to be significantly stronger compared with that in infected primary human hepatocytes and cholangiocytes. CK19 may be implicated in the pathogenesis of cholangiocarcinoma, as demonstrated by the stronger activity of its...Continue Reading

References

Oct 31, 1997·Lancet·S D Taylor-RobinsonH C Thomas
Mar 24, 2000·Trends in Genetics : TIG·D M NettelbeckR Müller
Dec 29, 2000·Seminars in Surgical Oncology·W R Jarnagin
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·K NakanoM Tanaka
Jun 8, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T Patel
Jun 30, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S C Afford, L S Young
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N HayashiM Monden
Mar 26, 2003·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Peter NagiMasato Yamamoto
May 25, 2004·Expert Opinion on Biological Therapy·Kirsi Saukkonen, Akseli Hemminki
Feb 4, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alphonse E Sirica
Sep 20, 2005·Journal of Hepatology·Michael Lie-A-LingPiter J Bosma

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