High protein/fish oil diet prevents hepatic steatosis in NONcNZO10 mice; association with diet/genetics-regulated micro-RNAs

Journal of Diabetes & Metabolism
Nikhil AdiKeith A Webster

Abstract

NONcNZO10 (NZ10) mice are predisposed to obesity and develop type 2 diabetes (T2D) and hepatic steatosis even when maintained on a control diet (CD) of 6% fat. Studies were designed to determine whether this extreme susceptibility phenotype could be alleviated by diet and if so the molecular targets of diet. NZ10 and SWR/J (SWR) control mice were fed a CD or a test diet of high protein and fish oil (HPO) for 19 weeks and then analyzed for steatosis, blood chemistry, hepatic gene and micro-RNA expression. HPO diet prevented steatosis, significantly increased serum adiponectin and reduced serum cholesterol and triglycerides only in NZ10 mice. The HPO diet repressed hepatic expression of fatty acid metabolic regulators including PPAR-γ, sterol regulatory element-binding protein-c1, peroxisome proliferator-activated receptor gamma co-activator-1, fatty acid synthase, fatty acid binding protein-4, and apolipoprotein A4 genes only in NZ10 mice. Also repressed by a HPO diet were adiponectinR2 receptor, leptin-R, PPAR-α, pyruvate dehydrogenase kinase isoforms 2 and 4, AKT2 and GSK3β. Micro-RNA (miR) arrays identified miRs that were diet and/or genetics regulated. QRTPCR confirmed increased expression of miR-205 and suppression of a ser...Continue Reading

Citations

Sep 12, 2018·Diseases·Brandon J PerumpailAijaz Ahmed
Jul 7, 2020·Journal of Diabetes Research·Linhao XuYizhou Xu
Jan 4, 2019·Molecular Diagnosis & Therapy·Lechun LyuXiang Li
May 24, 2018·Cellular and Molecular Life Sciences : CMLS·Amanda Karolina Soares Silva, Christina Alves Peixoto
Feb 16, 2021·Frontiers in Nutrition·Amira KohilAnnalisa Terranegra
Aug 25, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Mehdi ZobeiriMohammad Abdollahi

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