PMID: 25735343Mar 5, 2015Paper

High quality tissue miniarray technique using a conventional TV/radio telescopic antenna

Asian Pacific Journal of Cancer Prevention : APJCP
Mohamed A Elkablawy, Abdulkader M Albasri

Abstract

The tissue microarray (TMA) is widely accepted as a fast and cost-effective research tool for in situ tissue analysis in modern pathology. However, the current automated and manual TMA techniques have some drawbacks restricting their productivity. Our study aimed to introduce an improved manual tissue miniarray (TmA) technique that is simple and readily applicable to a broad range of tissue samples. In this study, a conventional TV/radio telescopic antenna was used to punch tissue cores manually from donor paraffin embedded tissue blocks which were pre-incubated at 40oC. The cores were manually transferred, organized and attached to a standard block mould, and filled with liquid paraffin to construct TmA blocks without any use of recipient paraffin blocks. By using a conventional TV/radio antenna, it was possible to construct TmA paraffin blocks with variable formats of array size and number (2-mm x 42, 2.5-mm x 30, 3-mm x 24, 4-mm x 20 and 5-mm x 12 cores). Up to 2-mm x 84 cores could be mounted and stained on a standard microscopic slide by cutting two sections from two different blocks and mounting them beside each other. The technique was simple and caused minimal damage to the donor blocks. H and E and immunostained slides...Continue Reading

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Citations

Oct 21, 2016·Journal of Biochemical and Molecular Toxicology·Nishat AhmedDina S El-Agamy
Jan 28, 2017·Human & Experimental Toxicology·D S El-AgamyA A Shaaban
Mar 24, 2017·PloS One·Hany M Abo-HadedDina S El-Agamy
Apr 3, 2016·Experimental Biology and Medicine·Dina S El-AgamyMohamed A Elkablawy
Dec 3, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Mohamed A ElkablawyAhmed Alhujaily

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