PMID: 7615556Jul 21, 1995

High rates of fatty acid oxidation during reperfusion of ischemic hearts are associated with a decrease in malonyl-CoA levels due to an increase in 5'-AMP-activated protein kinase inhibition of acetyl-CoA carboxylase

The Journal of Biological Chemistry
N KudoG D Lopaschuk


We determined whether high fatty acid oxidation rates during aerobic reperfusion of ischemic hearts could be explained by a decrease in malonyl-CoA levels, which would relieve inhibition of carnitine palmitoyl-transferase 1, the rate-limiting enzyme involved in mitochondrial uptake of fatty acids. Isolated working rat hearts perfused with 1.2 mM palmitate were subjected to 30 min of global ischemia, followed by 60 min of aerobic reperfusion. Fatty acid oxidation rates during reperfusion were 136% higher than rates seen in aerobically perfused control hearts, despite the fact that cardiac work recovered to only 16% of pre-ischemic values. Neither the activity of carnitine palmitoyltransferase 1, or the IC50 value of malonyl-CoA for carnitine palmitoyl-transferase 1 were altered in mitochondria isolated from aerobic, ischemic, or reperfused ischemic hearts. Levels of malonyl-CoA were extremely low at the end of reperfusion compared to levels seen in aerobic controls, as was the activity of acetyl-CoA carboxylase, the enzyme which produces malonyl-CoA. The activity of 5'-AMP-activated protein kinase, which has been shown to phosphorylate and inactivate acetyl-CoA carboxylase in other tissues, was significantly increased at the end...Continue Reading


Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D G Hardie, S A Hawley
Sep 11, 2001·Biochemical and Biophysical Research Communications·S L ChoiJ Ha
Dec 25, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·L DehinaQuadiri Timour
Jul 26, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Hiroyuki KageyamaNagara Tamaki
Dec 1, 2006·Cardiovascular Drugs and Therapy·G D Lopaschuk, William C Stanley
Dec 25, 2007·Cardiovascular Drugs and Therapy·Fanny VaillantQuadiri Timour
Feb 3, 2005·Current Atherosclerosis Reports·Clifford D L FolmesG D Lopaschuk
Nov 5, 2003·Current Hypertension Reports·R R Russell
Nov 8, 2006·Current Hypertension Reports·R R Russell
Dec 5, 2006·Cardiovascular Research·Teresa A HopkinsKenneth Walsh
Feb 11, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Toshiyuki FukaoGrant A Mitchell
Aug 4, 1997·The American Journal of Cardiology·G D Lopaschuk
Sep 2, 1999·Advances in Enzyme Regulation·M R Munday, C J Hemingway
Apr 12, 2003·Progress in Lipid Research·Nandakumar Sambandam, G D Lopaschuk
Mar 24, 1999·Trends in Biochemical Sciences·B E KempL A Witters
Oct 27, 1997·Journal of Pharmacological and Toxicological Methods·R L Barr, G D Lopaschuk
Jul 11, 2000·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·L H YoungR R Russell
Jun 17, 2000·Biochimica Et Biophysica Acta·J Kerner, C Hoppel
Nov 12, 2010·The Proceedings of the Nutrition Society·D G Hardie
Aug 17, 2010·Acta Pharmacologica Sinica·Najeeb A Shirwany, Ming-Hui Zou
Nov 17, 2009·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Daisuke Nagata, Yasunobu Hirata
Sep 18, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·G D Lopaschuk
Oct 17, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·O J RiderS Neubauer
Nov 24, 2004·Nature Medicine·Rei ShibataKenneth Walsh
Aug 3, 2004·Nature Reviews. Drug Discovery·Yuguang Shi, Paul Burn
May 7, 1999·The Biochemical Journal·N VoilleyM Prentki
Aug 6, 2000·The Biochemical Journal·C Hamilton, E D Saggerson
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·J HaK H Kim
Oct 29, 2009·The Journal of Biological Chemistry·Pernille R JensenMathilde H Lerche
May 22, 2010·The Journal of Biological Chemistry·Koji OhashiKenneth Walsh
Aug 24, 2010·The Journal of Biological Chemistry·Jaroslaw W ZmijewskiEdward Abraham
Nov 8, 2008·The Journal of Biological Chemistry·Jérome TerrandPhilippe Boucher


Sep 1, 1978·The American Journal of Cardiology·H S Mueller, S M Ayres
Oct 21, 1992·Molecular and Cellular Biochemistry·G D Lopaschuk, M Saddik
Nov 1, 1991·Analytical Biochemistry·D Constantin-TeodosiuE Hultman
Oct 1, 1991·Circulation Research·Y OhgoshiH Suga
Mar 10, 1990·European Journal of Biochemistry·R T MalletR Bünger
Sep 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K H KimM E Pape
May 1, 1989·Diabetes/metabolism Reviews·J D McGarryD W Foster
Oct 1, 1989·Circulation Research·B RenstromA J Liedtke
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S Svoronos, S Kumar
Oct 1, 1986·Biochemical Society Transactions·P J Randle
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Feb 1, 1966·The American Journal of Physiology·D R Challoner, D Steinberg
Jan 1, 1983·Annual Review of Biochemistry·S J WakilV C Joshi
Oct 1, 1994·Canadian Journal of Physiology and Pharmacology·G D Lopaschuk, J Gamble
Jul 1, 1994·American Heart Journal·G D LopaschukB A Finegan
Aug 4, 1994·Biochimica Et Biophysica Acta·G D LopaschukB O Schönekess
Feb 1, 1994·The Journal of Nutrition·H Schulz
Apr 1, 1965·Physiological Reviews·R J BING
Jan 1, 1974·Annual Review of Physiology·J R Neely, H E Morgan

Related Concepts

Fatty Acid Oxidation
Carnitine O-Palmitoyltransferase
Malonyl Coenzyme A
Myocardial Ischemia
Acetyl-CoA Carboxylase
Saturated Fat

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.