PMID: 7515970Jul 1, 1994Paper

High rates of frameshift mutations within homo-oligomeric runs during a single cycle of retroviral replication

Journal of Virology
D P Burns, H M Temin

Abstract

Homo-oligomeric runs were inserted into a spleen necrosis virus-based retrovirus vector to determine the nature and rate of mutations within runs of 10 to 12 identical nucleotides during a single replication cycle. Clones of helper cells containing integrated copies of retroviral vectors were used to produce virus for infection of target (nonhelper) cells. Proviral sequences from target cell clones were compared with proviral sequences from helper cell clones to study mutations that occurred during a single cycle of replication. In addition to the internal region spanning the homo-oligomeric inserts, a naturally occurring run of 10 T's in the long terminal repeat (LTR) also was sequenced. Rates of mutation ranged from < 0.01 to 0.38 frameshift mutations per run per cycle for different nucleotide runs. Frameshift mutations ranged from deletions of 2 bases to additions of 5 bases; the most common mutations were +1 and -1. Frameshift mutation rates did not increase as the run length increased from 10 to 12 bases. Rates of frameshift mutation for runs of T's and A's were significantly higher than rates for runs of C's and G's, and rates for runs of pyrimidines were significantly higher than those for runs of purines. Interestingly,...Continue Reading

References

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Citations

Mar 1, 1996·AIDS Research and Human Retroviruses·L M Mansky
Sep 25, 1999·The Journal of Biological Chemistry·M WisniewskiR A Bambara
Jun 30, 1995·Annals of the New York Academy of Sciences·H M Temin
Aug 7, 2003·Endothelium : Journal of Endothelial Cell Research·Raffaella Dell'EvaDouglas Noonan
Aug 12, 2003·Biotechnology and Bioengineering·Christina ValkovaGeorgi Milchev
Jun 3, 2005·Nature Reviews. Genetics·Jochen GrawRainer Schwaab
Jun 10, 2010·International Journal of Hematology·Chur-Woo YouHaing-Woon Baik
Jul 4, 2001·The Journal of Biological Chemistry·M BalakrishnanR A Bambara
Aug 22, 2001·Cadernos de saúde pública·M A OliveiraJ M Mello
Jul 28, 2010·Journal of Virology·Michael E AbramStephen H Hughes
Apr 15, 2003·The Journal of General Virology·Pablo de Felipe, Marta Izquierdo
Apr 7, 1998·Virology·D P WooleyR A Smith
Jan 1, 1996·Trends in Microbiology·B D Preston, J P Dougherty

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