Abstract
Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the causative agent of scrub typhus in East Asia. The genome sequence of this species has revealed an unprecedented number of repeat sequences, most notably of the genes encoding the conjugative properties of a type IV secretion system (T4SS). Although this observation is consistent with frequent intragenomic recombination, the extent of homologous recombination (gene conversion) in this species is unknown. To address this question, and to provide a protocol for the epidemiological surveillance of this important pathogen, we have developed a multilocus sequence typing (MLST) scheme based on 7 housekeeping genes (gpsA, mdh, nrdB, nuoF, ppdK, sucD, sucB). We applied this scheme to the two published genomes, and to DNA extracted from blood taken from 84 Thai scrub typhus patients, from 20 cultured Thai patient isolates, 1 Australian patient sample, and from 3 cultured type strains. These data demonstrated that the O. tsutsugamushi population was both highly diverse [Simpson's index (95% CI) = 0.95 (0.92-0.98)], and highly recombinogenic. These results are surprising given the intracellular life-style of this species, but are...Continue Reading
References
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1982·The American Journal of Tropical Medicine and Hygiene·A ShiraiE Gan
Nov 25, 1994·Science·D S Guttman, D E Dykhuizen
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C MaidenB G Spratt
Nov 11, 1999·Molecular Biology and Evolution·E J FeilB G Spratt
Apr 4, 2000·Genetics·E J FeilB G Spratt
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Oct 6, 2001·Current Opinion in Microbiology·B G SprattE J Feil
Oct 30, 2001·Journal of Clinical Microbiology·H GrundmannG Tanner
Jun 16, 2004·Current Opinion in Microbiology·Edward J Feil, Mark C Enright
Apr 1, 1997·Parasitology Today·R Rosenberg
Sep 21, 2004·Bioinformatics·D P MartinD Posada
Nov 4, 2005·Molecular Biology and Evolution·Laura BaldoJohn H Werren
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nam-Hyuk ChoIk-Sang Kim
Nov 23, 2007·Research in Microbiology·Hans-Henrik FuxeliusSiv G E Andersson
Mar 4, 2008·FEMS Immunology and Medical Microbiology·Stuart D BlacksellNicholas P J Day
Dec 19, 2008·Journal of Clinical Microbiology·Piengchan SonthayanonSharon J Peacock
Dec 23, 2008·Applied and Environmental Microbiology·Vera I D RosJohannes A J Breeuwer
May 20, 2009·BMC Bioinformatics·Alexandre P FranciscoJoão A Carriço
Citations
May 6, 2019·Tropical Medicine and Infectious Disease·Daryl J KellyAllen L Richards
Oct 2, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tri WangrangsimakulNicholas P J Day
Feb 21, 2014·Revista Da Sociedade Brasileira De Medicina Tropical·Luciana Inácia GomesAna Rabello
Mar 2, 2017·PLoS Neglected Tropical Diseases·Gwanghun KimNam-Hyuk Cho
Mar 11, 2017·PLoS Neglected Tropical Diseases·Nicole L MendellDavid H Walker
Feb 26, 2014·Emerging Infectious Diseases·Meng ZhangLi-Ping Yang
Dec 8, 2017·PLoS Pathogens·Jeanne Salje
Apr 14, 2015·Future Microbiology·Alison Luce-FedrowAllen L Richards
Oct 18, 2016·Frontiers in Cellular and Infection Microbiology·Nídia CangiNathalie Vachiéry
Jun 7, 2018·PLoS Neglected Tropical Diseases·Elizabeth M BattyJeanne Salje
Oct 3, 2018·Tropical Medicine and Infectious Disease·Daryl J KellyAllen L Richards
Jul 24, 2018·Microbial Genomics·Amy FleshmanTalima Pearson
Jul 18, 2018·Emerging Infectious Diseases·Nhiem Le-VietPhilippe Parola
Oct 3, 2018·Tropical Medicine and Infectious Disease·Alison Luce-FedrowAllen L Richards
Sep 10, 2020·Frontiers in Cellular and Infection Microbiology·Jiali LongZhicong Yang
Oct 11, 2013·Vector Borne and Zoonotic Diseases·Satoru AraiKazunori Oishi
Dec 4, 2013·The American Journal of Tropical Medicine and Hygiene·Ratree TakhampunyaJason H Richardson
Jan 15, 2017·BMC Infectious Diseases·Ranjan PremaratnaH J de Silva
Sep 1, 2015·PLoS Neglected Tropical Diseases·Rattanaphone PhetsouvanhNicholas P J Day
Jul 17, 2018·PLoS Neglected Tropical Diseases·Ratree TakhampunyaSilas A Davidson
Dec 14, 2017·Molecular Microbiology·Elisabeth GrohmannSteffen Backert
Nov 7, 2019·Parasites & Vectors·Ivo ElliottPaul N Newton
Jan 16, 2016·Vector Borne and Zoonotic Diseases·Kozue HottaDaisuke Hayasaka
Oct 31, 2020·Tropical Medicine and Infectious Disease·Daniel H ParisAllen L Richards
Mar 28, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ivo ElliottElizabeth M Batty
May 1, 2021·Pathogens·Rajendra GautamJeevan Bahadur Sherchand
Sep 22, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Veasna DuongPhilippe Buchy
Jan 19, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Veasna DuongPhilippe Buchy
Jun 30, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Patimaporn WongprompitakYupin Suputtamongkol