High rates of homologous recombination in the mite endosymbiont and opportunistic human pathogen Orientia tsutsugamushi.

PLoS Neglected Tropical Diseases
Piengchan SonthayanonNicholas P J Day

Abstract

Orientia tsutsugamushi is an intracellular alpha-proteobacterium which resides in trombiculid mites, and is the causative agent of scrub typhus in East Asia. The genome sequence of this species has revealed an unprecedented number of repeat sequences, most notably of the genes encoding the conjugative properties of a type IV secretion system (T4SS). Although this observation is consistent with frequent intragenomic recombination, the extent of homologous recombination (gene conversion) in this species is unknown. To address this question, and to provide a protocol for the epidemiological surveillance of this important pathogen, we have developed a multilocus sequence typing (MLST) scheme based on 7 housekeeping genes (gpsA, mdh, nrdB, nuoF, ppdK, sucD, sucB). We applied this scheme to the two published genomes, and to DNA extracted from blood taken from 84 Thai scrub typhus patients, from 20 cultured Thai patient isolates, 1 Australian patient sample, and from 3 cultured type strains. These data demonstrated that the O. tsutsugamushi population was both highly diverse [Simpson's index (95% CI) = 0.95 (0.92-0.98)], and highly recombinogenic. These results are surprising given the intracellular life-style of this species, but are...Continue Reading

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Citations

May 6, 2019·Tropical Medicine and Infectious Disease·Daryl J KellyAllen L Richards
Oct 2, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tri WangrangsimakulNicholas P J Day
Feb 21, 2014·Revista Da Sociedade Brasileira De Medicina Tropical·Luciana Inácia GomesAna Rabello
Mar 11, 2017·PLoS Neglected Tropical Diseases·Nicole L MendellDavid H Walker
Feb 26, 2014·Emerging Infectious Diseases·Meng ZhangLi-Ping Yang
Apr 14, 2015·Future Microbiology·Alison Luce-FedrowAllen L Richards
Oct 18, 2016·Frontiers in Cellular and Infection Microbiology·Nídia CangiNathalie Vachiéry
Oct 3, 2018·Tropical Medicine and Infectious Disease·Daryl J KellyAllen L Richards
Jul 18, 2018·Emerging Infectious Diseases·Nhiem Le-VietPhilippe Parola
Oct 3, 2018·Tropical Medicine and Infectious Disease·Alison Luce-FedrowAllen L Richards
Sep 10, 2020·Frontiers in Cellular and Infection Microbiology·Jiali LongZhicong Yang
Oct 11, 2013·Vector Borne and Zoonotic Diseases·Satoru AraiKazunori Oishi
Sep 1, 2015·PLoS Neglected Tropical Diseases·Rattanaphone PhetsouvanhNicholas P J Day
Dec 14, 2017·Molecular Microbiology·Elisabeth GrohmannSteffen Backert
Nov 7, 2019·Parasites & Vectors·Ivo ElliottPaul N Newton
Jan 16, 2016·Vector Borne and Zoonotic Diseases·Kozue HottaDaisuke Hayasaka
Oct 31, 2020·Tropical Medicine and Infectious Disease·Daniel H ParisAllen L Richards
Mar 28, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ivo ElliottElizabeth M Batty
May 1, 2021·Pathogens·Rajendra GautamJeevan Bahadur Sherchand
Sep 22, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Veasna DuongPhilippe Buchy
Jan 19, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Veasna DuongPhilippe Buchy
Jun 30, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Patimaporn WongprompitakYupin Suputtamongkol

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SiScan
Geneconv
eBURST
MEGA
BLASTN
Chimaera
3Seq
Max
START
goeBURST

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