High reliability and sensitivity of the BCR/ABL1 D-FISH test for the detection of BCR/ABL rearrangements

Annals of Hematology
A F PelzM Stumm

Abstract

The BCR/ABL1 fusion gene is mainly caused by the t(9; 22)(q34; q11.2) translocation, which results in the Philadelphia (Ph) chromosome. The Ph chromosome is the typical hallmark in chronic myeloid leukemia (CML), but can also be present in acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). The BCR/ABL1 rearrangement is an important tumor classification marker and a useful prognostic factor allowing an adequate therapy management. Ph chromosome detection by conventional cytogenetics (CC) can be hampered by low quantity and quality of metaphases from tumor cells. Furthermore, BCR/ABL1 rearrangements may be hidden due to cryptic rearrangements or complex aberrations. Therefore, molecular cytogenetic methods turned out to be useful tools for the detection of BCR/ABL1 rearrangements. We performed fluorescent in situ hybridization (FISH) with the recently developed BCR/ABL1 D-FISH probe (QBIOgene, Illkirch, F) on cultured bone marrow and peripheral blood cells of 71 patients with CML, ALL, AML, and myeloproliferative disorder (MPD). FISH results and the results of banding methods were directly compared. Based on the analyses of >200 nuclei per patient, D-FISH correlated closely with CC and allowed an accurate quanti...Continue Reading

Citations

Feb 22, 2005·Cancer Genetics and Cytogenetics·Antje-Friederike PelzPeter Wieacker
Oct 29, 2003·Cancer Genetics and Cytogenetics·Sabine Naumann, Hans-Jochen Decker
Apr 23, 2003·The Journal of Molecular Diagnostics : JMD·Amgad L NashedMargaret L Gulley
Jan 15, 2014·Leukemia & Lymphoma·Fausto R LoberizaPhilip J Bierman
Mar 27, 2007·The Journal of Molecular Diagnostics : JMD·Zsolt JobbagyChristopher D Gocke
Aug 16, 2005·British Journal of Haematology·Louise K Jones, Vaskar Saha
Sep 2, 2011·Pathology·David T YeungSusan Branford
Dec 14, 2004·Cancer Genetics and Cytogenetics·Antje-Friederike PelzPeter F Wieacker

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