PMID: 9545599Apr 18, 1998Paper

High resolution 1H NMR spectroscopic studies on dynamic biochemical processes in incubated human seminal fluid samples

Biochimica Et Biophysica Acta
A M TomlinsJeremy K Nicholson

Abstract

High resolution 600 MHz 1H NMR spectroscopy was used to investigate the changes in biochemical composition of whole human seminal fluid (SF) and an artificial mixture of prostatic (PF) and seminal vesicle fluid (SVF). A variety of time-related biochemical changes were monitored simultaneously and non-invasively in SF, including enzymatic hydrolysis of phosphorylcholine to choline and polypeptides to amino acids. The fastest NMR-observable reactions in SF were the conversion of phosphorylcholine to choline (t1/2 approximately equal to 9 min) and uridine-5'-monophosphate (UMP) to uridine (t1/2 < 2 min). UMP has not previously been detected in SF because of its rapid hydrolysis. Artificial mixtures of separately obtained prostatic and SVF showed very similar biochemical changes to those observed in whole SF. Addition of EDTA to SF incubated for 2 min post ejaculation strongly inhibited peptide hydrolysis. Zn2+, present in whole SF was shown to be non EDTA-chelatable 2 min after ejaculation, whereas after 7 min, a singlet signal from the ethylenic protons of the Zn-EDTA2- complex was clearly observed which remained constant after 7 min. This indicates that soon after ejaculation (< 5 min) Zn2+ is immobilised in a macromolecular com...Continue Reading

Citations

Jul 4, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Elaine HolmesSarah J Tabrizi
Jul 11, 2012·Cell and Tissue Research·Maria Manuela FrancouJoaquín De Juan
Jun 27, 2014·Journal of Assisted Reproduction and Genetics·Varshini JayaramanPratap Kumar Narayan
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Ashish GuptaSatya Narain Shankhwar
Sep 17, 2009·NMR in Biomedicine·Nicholas G SpencerGeoffrey S Payne
Jun 14, 2013·NMR in Biomedicine·Thiele KobusArend Heerschap
Mar 10, 2015·Computational and Structural Biotechnology Journal·Jeremy R Everett
Apr 24, 2008·Nature Clinical Practice. Gastroenterology & Hepatology·Jacob T BjerrumJørgen Olsen
Jul 18, 2002·Nature Reviews. Drug Discovery·Jeremy K NicholsonElaine Holmes
Apr 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T M TsangE Holmes
Feb 11, 2015·Applied and Environmental Microbiology·Francisco J AlvarezPer O Ljungdahl
Nov 16, 2012·Nature·Jeremy K NicholsonJohn C Lindon
Jun 6, 2013·Magnetic Resonance in Chemistry : MRC·Paola ScanoErnesto d'Aloja
Aug 4, 2015·Systems Biology in Reproductive Medicine·Claudia BonechiClaudio Rossi
Nov 4, 2020·Open Biology·Gitanjali AsampilleHanudatta S Atreya
Jan 4, 2008·Chemical Research in Toxicology·Muireann CoenJeremy K Nicholson
Dec 18, 2021·Magma·Marc JupinArend Heerschap

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.