PMID: 1747Dec 1, 1975

High resolution 31P nuclear magnetic resonance studies of intact yeast cells

Proceedings of the National Academy of Sciences of the United States of America
J M SalhanyS Ogawa

Abstract

High resolution 31P nuclear magnetic resonance (NMR) spectra at 145.7 MHZ are presented for intact yeast cells. Several peaks are resolved and assigned. They include the middle phosphate peaks from long chain or cyclic polyphosphates. Our results are consistent with the suggestion that these polyphosphates act as a phosphate store in the cell. We have also been able to measure cytoplasmic pH using the orthophosphate peak inside the cell, as compared with outside the cell. The results show that yeast cells maintain their cytoplasmic pH around 6.3. This value is considerably higher than the acidic extracellular pH at which they normally live. These preliminary results indicate that 31P NMR at 145.7 MHZ can be a rapid, informative, and non-invasive method for probing biochemical events within living cells.

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Guéron, R G Shulman
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·T O HendersonA Omachi
May 1, 1963·The Journal of General Physiology·A ROTHSTEIN

Citations

Jan 29, 2003·Journal of Cellular Biochemistry. Supplement·R J Gillies
Dec 1, 1992·Journal of Bioenergetics and Biomembranes·T A Krulwich, A A Guffanti
Oct 1, 2013·Calcified Tissue International·Sidney OmelonAntonio Nanci
Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Jan 1, 1984·The International Journal of Biochemistry·G Matheis, J R Whitaker
Dec 20, 1982·Life Sciences·C T Burt
Jul 22, 1982·Biochimica Et Biophysica Acta·A L DingleyM F Roberts
Nov 9, 1981·Biochimica Et Biophysica Acta·J K Roberts, O Jardetzky
Dec 30, 1981·Biochimica Et Biophysica Acta·A Goffeau, C L Slayman
Jul 26, 2002·Archives of Biochemistry and Biophysics·Man Zhang, Jon D Robertus
Oct 20, 2005·Journal of Bioscience and Bioengineering·I KulaevT Kulakovskaya
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G NavonT Yamane
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S M Cohen, C T Burt
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F E Evans, N O Kaplan
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G NavonT Yamane
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A Lapidot, C S Irving
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B ChanceG G McDonald
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G NavonT Yamane
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UgurbilR G Shulman
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K UgurbilR G Shulman
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T H GroveP J Bore
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J K BartonR G Shulman
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E T FosselJ S Ingwall
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R J GilliesR G Shulman
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M S SwansonR S Markin
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Kanodia, M F Roberts
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T A GlaserA J Mukkada
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xiaomin YuWilliam W Metcalf
Dec 1, 1981·Biochimica Et Biophysica Acta·G W Borst-Pauwels
Sep 27, 1984·Biochimica Et Biophysica Acta·J Slavík, A Kotyk
Feb 7, 1977·Biochemical and Biophysical Research Communications·P B GarlickP J Seeley
May 31, 1982·Biochemical and Biophysical Research Communications·T MatsunagaS Toda
Jul 1, 1981·Analytical Biochemistry·J S TaylorD F Wilson
Aug 14, 1980·Biochemical and Biophysical Research Communications·R DeslauriersI C Smith
Apr 1, 1982·Analytical Biochemistry·D L Rabenstein, A A Isab
Nov 1, 1985·Survey of Ophthalmology·J V GreinerT Glonek
Nov 18, 2005·Protein Expression and Purification·Ronald T Niebauer, Anne S Robinson
Jan 1, 1983·Critical Reviews in Oncology/hematology·J A Koutcher
Jul 11, 1977·Biochemical and Biophysical Research Communications·P A Sehr, G K Radda
Sep 15, 1987·Biochemical and Biophysical Research Communications·C DucrocqJ C Beloeil
Feb 28, 1979·Biochemical and Biophysical Research Communications·J M SalhanyJ M Hassing
Dec 1, 1978·Archives of Biochemistry and Biophysics·F C Womack, S P Colowick
Apr 2, 1980·Biochimica Et Biophysica Acta·S OgawaC L Castillo
Dec 4, 1978·Biochimica Et Biophysica Acta·M C BlokB de Kruijff
Oct 7, 2006·Biotechnology Progress·Alison WedekindAnne S Robinson
Sep 4, 2015·BMC Genomics·Katharina PapsdorfKlaus Richter
Feb 1, 1981·Bioscience Reports·L Jacobson, J S Cohen
Feb 23, 2002·Protein Expression and Purification·Valérie SarramegnaFranck Talmont
Mar 15, 1984·Biochemical and Biophysical Research Communications·J P Tijssen, J Van Steveninck
Jan 14, 1986·Biochemistry·J A den HollanderR G Shulman
Aug 25, 2006·Biochimica Et Biophysica Acta·Dipankar MalakarAnil K Ghosh
Oct 29, 1992·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A BertlC L Slayman
Dec 1, 1980·Journal of Cellular Physiology·H Inoue, T Yoshioka
May 1, 1981·European Journal of Biochemistry·K NicolayW N Konings
May 1, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·George S BiggsPaul D Barker
Apr 17, 2020·ChemPlusChem·Siddarth NarasimhanMarc Baldus
Jan 1, 1978·CRC Critical Reviews in Biochemistry·J S Cohen
Nov 16, 1987·FEBS Letters·P Eraso, C Gancedo
Oct 1, 1988·International Journal of Radiation Biology·P K HolahanC E Swenberg
Jan 26, 2021·Neuroscience·Hahnsung KimPhillip Zhe Sun

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