DOI: 1909.00023Sep 9, 2019Paper

High-resolution 3D refractive index microscopy of multiple-scattering samples from intensity images

ArXiv
Shwetadwip ChowdhuryLaura Waller

Abstract

Optical diffraction tomography (ODT) reconstructs a samples volumetric refractive index (RI) to create high-contrast, quantitative 3D visualizations of biological samples. However, standard implementations of ODT use interferometric systems, and so are sensitive to phase instabilities, complex mechanical design, and coherent noise. Furthermore, their reconstruction framework is typically limited to weakly-scattering samples, and thus excludes a whole class of multiple-scattering samples. Here, we implement a new 3D RI microscopy technique that utilizes a computational multi-slice beam propagation method to invert the optical scattering process and reconstruct high-resolution (NA>1.0) 3D RI distributions of multiple-scattering samples. The method acquires intensity-only measurements from different illumination angles, and then solves a non-linear optimization problem to recover the sample 3D RI distribution. We experimentally demonstrate reconstruction of samples with varying amounts of multiple scattering: a 3T3 fibroblast cell, a cluster of C. elegans embryos, and a whole C. elegans worm, with lateral and axial resolutions of 250 nm and 900 nm, respectively.

Related Concepts

Related Feeds

BioHub - Researcher Network

The Chan-Zuckerberg Biohub aims to support the fundamental research and develop the technologies that will enable physicians to cure, prevent, or manage all diseases in our childrens' lifetimes. The CZ Biohub brings together researchers from UC Berkeley, Stanford, and UCSF. Find the latest research from the CZ Biohub researcher network here.