High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis.

Nature Biotechnology
Rupali P PatwardhanJay Shendure

Abstract

We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.

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Citations

Nov 30, 2010·Nature Biotechnology·Sriram KosuriGeorge M Church
Apr 26, 2011·Nature Biotechnology·Jiayuan QuanJingdong Tian
Mar 1, 2012·Nature Biotechnology·Rupali P PatwardhanJay Shendure
Jun 9, 2012·Nature Biotechnology·Vanja Haberle, Boris Lenhard
Jun 9, 2012·Nature Biotechnology·Shoshana J Wodak
Nov 10, 2012·Nature Biotechnology·Jay Shendure, Erez Lieberman Aiden
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