High-resolution analysis of the human T-cell receptor repertoire

Nature Communications
Eliana RuggieroChristof von Kalle

Abstract

Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies TCR α- and β-chain diversity without sequence-associated or quantitative restrictions in healthy and diseased conditions. TCR-LA-MC PCR identifies convergent recombination events, classifies different stages of cutaneous T-cell lymphoma in vivo and demonstrates TCR reactivation after in vitro cytomegalovirus stimulation. TCR-LA-MC PCR allows ultra-deep data access to both physiological TCR diversity and mechanisms influencing clonality in all clinical settings with restricted or distorted TCR repertoires.

Associated Clinical Trials

Nov 10, 2020·Maria Chiara Bonini

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Datasets Mentioned

BETA
SRP059581

Methods Mentioned

BETA
PCR
454 sequencing
Illumina sequencing
FACS

Software Mentioned

BLAT
BLAST
MiTCR

Related Concepts

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