High resolution and rapid analysis of branched oligosaccharides by capillary electrophoresis

Analytical Biochemistry
P CamilleriG Okafo

Abstract

The fluorophore 2-aminoacridone has been used to label a number of branched oligosaccharides previously released from various glycoproteins. Complex glycans were derivatized via their reducing end with this fluorophore by a Schiff's base mechanism followed by reduction to a secondary amine using sodium cyanoborohydride. This process of derivatization was carried out efficiently and in a nonselective manner over a period of 30 min at 90 degrees C. The resulting derivatives were separated with high resolution by capillary electrophoresis using borate buffer, containing taurodeoxycholate, as the separation buffer. The method described does not require the removal of sialic acid residues prior to derivatization, so that treatment of glycans with N-acetyl neuraminidase provided useful and additional structural information. Attempts have also been made to relate the electrophoretic mobility of branched oligosaccharides with their molecular volume.

Citations

Mar 13, 2010·Analytical and Bioanalytical Chemistry·L R RuhaakM Wuhrer
Nov 1, 2008·Mass Spectrometry Reviews·Yehia Mechref, Milos V Novotny
Apr 9, 2013·Analytical Chemistry·Stefan MittermayrAndrás Guttman
Feb 14, 2002·Chemical Reviews·Yehia Mechref, Milos V Novotny
Jul 17, 1999·Rapid Communications in Mass Spectrometry : RCM·J CharlwoodP Camilleri
Mar 10, 2001·Rapid Communications in Mass Spectrometry : RCM·J Delaney, P Vouros
May 24, 2006·Electrophoresis·Satoru Kamoda, Kazuaki Kakehi
Apr 2, 2005·Rapid Communications in Mass Spectrometry : RCM·Willy MorelleJean-Claude Michalski
Feb 1, 1996·Electrophoresis·Z El Rassi, Y Mechref
Jul 22, 2009·Electrophoresis·Isabella RustighiAmelia Gamini
Feb 10, 1999·Rapid Communications in Mass Spectrometry : RCM·J CharlwoodP Camilleri
Feb 10, 1998·Electrophoresis·H A BardelmeijerW J Underberg
Dec 16, 1998·Electrophoresis·S Suzuki, S Honda
Aug 1, 1997·Current Opinion in Biotechnology·P M Rudd, R A Dwek
Nov 7, 2003·Journal of Chromatography. a·Erika Lattova, Hélène Perreault
Dec 31, 2003·Bioorganic & Medicinal Chemistry Letters·Mamoru MizunoToshiyuki Inazu
Jul 17, 1998·Analytical Chemistry·J CharlwoodP Camilleri
Apr 14, 2000·Analytical Chemistry·J CharlwoodP Camilleri
Apr 14, 2000·Analytical Chemistry·J CharlwoodP Camilleri

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