High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells.

Molecular & Cellular Proteomics : MCP
Veronika KanderovaTomas Kalina

Abstract

Acute leukemia is a disease pathologically manifested at both genomic and proteomic levels. Molecular genetic technologies are currently widely used in clinical research. In contrast, sensitive and high-throughput proteomic techniques for performing protein analyses in patient samples are still lacking. Here, we used a technology based on size exclusion chromatography followed by immunoprecipitation of target proteins with an antibody bead array (Size Exclusion Chromatography-Microsphere-based Affinity Proteomics, SEC-MAP) to detect hundreds of proteins from a single sample. In addition, we developed semi-automatic bioinformatics tools to adapt this technology for high-content proteomic screening of pediatric acute leukemia patients.To confirm the utility of SEC-MAP in leukemia immunophenotyping, we tested 31 leukemia diagnostic markers in parallel by SEC-MAP and flow cytometry. We identified 28 antibodies suitable for both techniques. Eighteen of them provided excellent quantitative correlation between SEC-MAP and flow cytometry (p< 0.05). Next, SEC-MAP was applied to examine 57 diagnostic samples from patients with acute leukemia. In this assay, we used 632 different antibodies and detected 501 targets. Of those, 47 targets w...Continue Reading

References

Oct 1, 1979·Analytical Biochemistry·G J Dimitriadis
Jun 1, 1990·Journal of Biochemical and Biophysical Methods·I Hanukoglu
Jul 3, 2002·Leukemia·O Hrusák, A Porwit-MacDonald
Apr 1, 2003·Molecular and Cellular Biology·Daniela BarilàRoberto Testi
Jul 2, 2003·The Journal of Experimental Medicine·John A KellyWarren J Leonard
Jul 9, 2004·Molecular & Cellular Proteomics : MCP·Qiang TianLeroy E Hood
Dec 25, 2004·Leukemia·D-C LiangW-H Chang
Aug 30, 2005·Journal of Immunological Methods·Larissa BelovRichard I Christopherson
Apr 11, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·John P Nolan, Francis Mandy
May 12, 2006·Electrophoresis·Angela Dittmer, Jürgen Dittmer
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring
Aug 19, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wenjing Ruan, Maode Lai
Sep 18, 2008·Molecular & Cellular Proteomics : MCP·Weiwei WuFridtjof Lund-Johansen
Oct 9, 2008·Blood·Steven M KornblauGordon B Mills
Feb 3, 2009·Immunology Letters·Karel SvojgrJan Zuna
Feb 25, 2009·Nucleic Acids Research·J M RuijterA F M Moorman
Sep 18, 2009·Pediatric Blood & Cancer·Ester MejstríkováOndrej Hrusák
Dec 18, 2009·Leukemia·J StaryUNKNOWN Czech Pediatric Hematology working group
Dec 3, 2010·Best Practice & Research. Clinical Haematology·E DekkingUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Dec 4, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ching-Hon Pui
Dec 15, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Charles G Mullighan
Dec 21, 2010·Current Opinion in Pediatrics·Kathryn G Roberts, Charles G Mullighan
Dec 24, 2010·Science·UNKNOWN modENCODE ConsortiumManolis Kellis
Aug 16, 2011·New Biotechnology·Anders HolmFridtjof Lund-Johansen
Jan 4, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jan StuchlýTomáš Kalina
May 4, 2012·Leukemia·J J M van DongenUNKNOWN EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)

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