High-resolution array CGH of metanephric adenomas: lack of DNA copy number changes

Histopathology
Adrianna SzponarG Kovacs

Abstract

Previous karyotyping and fluorescence in situ hybridization analysis of metanephric adenomas (MAs) has yielded controversial data. The aim of this study was to detect small genomic alterations, if any, specific to MAs by applying high-resolution oligoarrays. DNA extracted from paraffin blocks of six metanephric adenomas was hybridized onto Agilent oligoarrays with approximately 43,000 in situ synthesized 60-mer oligonucleotide probes that span coding and non-coding sequences with an average spatial resolution of approximately 35 kb. None of the metanephric adenomas showed DNA copy number changes. To confirm our results, DNA extracted from the paraffin block of a chromophobe renal cell carcinoma (RCC) was simultaneously hybridized to one of the four arrays on the same slides as an internal control. The chromophobe RCC showed loss of several chromosomes but no alteration was seen in MAs. We have confirmed the negative results by dye-swap and sex mismatch hybridization experiments. Our high-resolution oligoarray analysis indicates that metanephric adenomas lack DNA copy number alterations. This finding may help to differentiate between metanephric adenomas from Wilms' tumour and papillary renal cell adenoma with overlapping phenot...Continue Reading

References

Jul 1, 1991·Genes, Chromosomes & Cancer·G KovacsH F Kung
Oct 1, 1995·The American Journal of Surgical Pathology·C J DavisF K Mostofi
Feb 1, 1997·Diagnostic Cytopathology·A A RenshawJ A Fletcher
Nov 14, 1997·American Journal of Clinical Pathology·S R GranterA A Renshaw
May 5, 1999·International Journal of Urology : Official Journal of the Japanese Urological Association·M TsujiS Kagawa
Apr 11, 2000·The American Journal of Surgical Pathology·A A RenshawY A Hammers
Oct 16, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Matteo BrunelliLiang Cheng
Mar 5, 2005·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Dinesh RakhejaLinda R Margraf
Aug 30, 2006·Human Pathology·Evelyne LerutMaria Debiec-Rychter
Nov 14, 2006·Journal of Clinical Pathology·Maximilian BurgerArndt Hartmann
Jan 7, 2009·International Journal of Cancer. Journal International Du Cancer·Adrianna SzponarGyula Kovacs

❮ Previous
Next ❯

Citations

Aug 31, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Chin-Chen Pan, Jonathan I Epstein
Jun 26, 2012·European Urology·Toni K ChoueiriSabina Signoretti
Apr 5, 2013·Histopathology·Mariana Mantoan PadilhaCristina Magi-Galluzzi
Oct 9, 2015·BioMed Research International·Ning Yi YapGlenda Carolyn Gobe
May 28, 2015·Human Pathology·Rose ChamiPaul Scott Thorner
May 20, 2021·Advances in Anatomic Pathology·Nicholas BaniakMichelle S Hirsch
Nov 14, 2020·Surgical Pathology Clinics·Amy L Treece

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Chin-Chen Pan, Jonathan I Epstein
Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Semra OlgacVictor E Reuter
The American Journal of Surgical Pathology
A A RenshawY A Hammers
© 2021 Meta ULC. All rights reserved