High-resolution atmospheric-pressure MALDI mass spectrometry imaging workflow for lipidomic analysis of late fetal mouse lungs

Scientific Reports
Vannuruswamy GarikapatiBernhard Spengler

Abstract

Mass spectrometry imaging (MSI) provides label-free, non-targeted molecular and spatial information of the biomolecules within tissue. Lipids play important roles in lung biology, e.g. as surfactant, preventing alveolar collapse during normal and forced respiration. Lipidomic characterization of late fetal mouse lungs at day 19 of gestation (E19) has not been performed yet. In this study we employed high-resolution atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization MSI for the lipidomic analysis of E19 mouse lungs. Molecular species of different lipid classes were imaged in E19 lung sections at high spatial and mass resolution in positive- and negative-ion mode. Lipid species were characterized based on accurate mass and on-tissue tandem mass spectrometry. In addition, a dedicated sample preparation protocol, homogenous deposition of matrices on tissue surfaces and data processing parameters were optimized for the comparison of signal intensities of lipids between different tissue sections of E19 lungs of wild type and Pex11β-knockout mice. Our study provides lipid information of E19 mouse lungs, optimized experimental and data processing strategies for the direct comparison of signal intensiti...Continue Reading

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Citations

Jun 24, 2020·Journal of Separation Science·Na LiHuwei Liu
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Apr 9, 2021·Analytical and Bioanalytical Chemistry·Amanda VaughnDean R Appling
May 1, 2021·Life·Jayan D M Senevirathna, Shuichi Asakawa
May 22, 2021·Chemical Reviews·Philip A DobleDavid Clases
Feb 8, 2020·Journal of the American Society for Mass Spectrometry·Hongxia BaiDavid C Muddiman
Nov 20, 2021·Pflügers Archiv : European journal of physiology·Pegah Khamehgir-SilzAndreas H Wagner
Dec 4, 2021·Journal of the American Society for Mass Spectrometry·Alexander KnodelJoachim Franzke
Feb 2, 2022·Journal of the American Society for Mass Spectrometry·Chao DongKermit K Murray

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Methods Mentioned

BETA
xenografts

Software Mentioned

MSiReader
imzML converter
SMALDIPrep
MetFrag
Image composite editor

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