High-resolution computer simulation of the dynamics of isoelectric focusing using carrier ampholytes: focusing with concurrent electrophoretic mobilization is an isotachophoretic process

Electrophoresis
Wolfgang Thormann, R A Mosher

Abstract

Focusing of four hemoglobins with concurrent electrophoretic mobilization was studied by computer simulation. A dynamic electrophoresis simulator was first used to provide a detailed description of focusing in a 100-carrier component, pH 6-8 gradient using phosphoric acid as anolyte and NaOH as catholyte. These results are compared to an identical simulation except that the catholyte contained both NaOH and NaCl. A stationary, steady-state distribution of carrier components and hemoglobins is produced in the first configuration. In the second, the chloride ion migrates into and through the separation space. It is shown that even under these conditions of chloride ion flux a pH gradient forms. All amphoteric species acquire a slight positive charge upon focusing and the whole pattern is mobilized towards the cathode. The cathodic gradient end is stable whereas the anodic end is gradually degrading due to the continuous accumulation of chloride. The data illustrate that the mobilization is a cationic isotachophoretic process with the sodium ion being the leading cation. The peak height of the hemoglobin zones decreases somewhat upon mobilization, but the zones retain a relatively sharp profile, thus facilitating detection. The el...Continue Reading

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Jan 27, 2004·Electrophoresis·Wolfgang ThormannRichard A Mosher

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Citations

Nov 13, 2007·Analytical Chemistry·Jacob W AlbrechtKlavs F Jensen
Nov 11, 2014·Proceedings. Mathematical, Physical, and Engineering Sciences·L V SakharovaM Yu Zhukov
Dec 8, 2009·Journal of Chromatography. a·Ying Chou, Ruey-Jen Yang
Mar 3, 2007·Journal of Chromatography. a·M HorkáK Slais
Jun 12, 2009·Electrophoresis·Wolfgang ThormannRichard A Mosher
Feb 5, 2008·Electrophoresis·Jia-Wei YuRuey-Jen Yang
Feb 28, 2009·Electrophoresis·Michael C BreadmoreWolfgang Thormann
Jan 22, 2009·Electrophoresis·Vlastimil HruskaGyula Vigh
Dec 25, 2008·Electrophoresis·Kiyohito Shimura
Feb 20, 2007·Journal of Separation Science·Jan PetrZdenek Stránský
Apr 3, 2008·Electrophoresis·Wolfgang Thormann, Richard A Mosher
Feb 10, 2009·Electrophoresis·Yin Chou, Ruey-Jen Yang
Oct 23, 2012·Electrophoresis·Csilla PágerFerenc Kilár
Dec 31, 2010·Electrophoresis·Jessica KösterGünther Weber
Nov 28, 2012·Proteomics·Pier Giorgio RighettiAttilio Citterio
Mar 2, 2010·Electrophoresis·Wolfgang ThormannRichard A Mosher
Jul 22, 2021·Electrophoresis·Wolfgang Thormann, Richard A Mosher
Apr 3, 2007·Journal of Proteome Research·Hoang-Trang LamH H Girault

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