High resolution DNA flow cytometry of boar sperm cells in identification of boars carrying cytogenetic aberrations

Theriogenology
Jacob LarsenIb Jarle Christensen

Abstract

The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resolution DNA flow cytometry on DAPI-stained sperm cell nuclei (CV </= 1.3%) was investigated as a screening method for the cytogenetic quality of boars. By analyzing a series of 25 animals judged normal by their fertility statistics and a series of seven animals with known reciprocal translocations, a model for identifying sperm cells from cytogenetically aberrant animals was proposed. This model was applied to a series of 50 uncharacterized animals. The model successfully identified a mosaic or chimaeric carrier of an aberrant X chromosome. However, implementation of this technique for screening purposes would necessitate essential improvements in standardization and measurement precision.

References

Jan 1, 1980·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·C C LinR B Church
Feb 5, 2000·Molecular Reproduction and Development·M HazzouriB Sèle
Jun 14, 2000·Animal Reproduction Science·L A Johnson
Jan 25, 2002·American Journal of Medical Genetics·Franck PellestorBrigitte Andréo

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