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High-resolution imaging of cytoplasmic Ca2+ dynamics in Arabidopsis roots

Cold Spring Harbor Protocols

Jul 3, 2013

Smrutisanjita Behera, Jörg Kudla

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Abstract

This protocol describes a method for imaging cytoplasmic Ca(2+) dynamics in roots with high resolution using confocal laser scanning microscopy (CLSM). The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, stably e...read more

Mentioned in this Paper

Cations, Divalent
Energy Transfer
Arabidopsis
Calcium
Ubiquitin
Protoplasm
Promoter
Plant bulb
Ubiquitin Activity
Gene Expression
Paper Details
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High-resolution imaging of cytoplasmic Ca2+ dynamics in Arabidopsis roots

Cold Spring Harbor Protocols

Jul 3, 2013

Smrutisanjita Behera, Jörg Kudla

PMID: 23818666

DOI: 10.1101/pdb.prot073023

Abstract

This protocol describes a method for imaging cytoplasmic Ca(2+) dynamics in roots with high resolution using confocal laser scanning microscopy (CLSM). The Förster (fluorescence) resonance energy transfer (FRET)-based genetically modified Ca(2+) indicator Yellow Cameleon YC3.6, stably e...read more

Mentioned in this Paper

Cations, Divalent
Energy Transfer
Arabidopsis
Calcium
Ubiquitin
Protoplasm
Promoter
Plant bulb
Ubiquitin Activity
Gene Expression

Feeds With Similar Papers

Ubiquitin System

Ubiquitin is a small regulatory protein that is attached to proteins as a post-translational modification in a process known as ubiquitination to regulate protein turnover, location, or activity. Here is the latest research on the ubiquitin system.

Related Papers

Cold Spring Harbor Protocols

Live cell imaging of cytoplasmic Ca2+ dynamics in Arabidopsis guard cells

Cold Spring Harbor ProtocolsJuly 3, 2013
Smrutisanjita Behera, Jörg Kudla
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  • Citations5
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