Abstract
The integrin alphavbeta6 is expressed only on epithelia and then usually only during processes of tissue remodelling including cancer, where its high expression correlates with reduced survival. Thus, alphavbeta6 represents an important target for imaging and therapy of cancer and new molecular-specific targeting agents are required. We have developed A20FMDV2, a peptide derived from the VP1 coat protein of foot-and-mouth-disease virus that binds specifically and stably to alphavbeta6. Using a newly generated pair of isogenic human cell lines that differ only in alphavbeta6 expression, it was shown, using biodistribution and SPECT imaging, that indium-111-labelled A20FMDV2 locates specifically to alphavbeta6-expressing tissues in vivo, achieving at least seven-times higher retention in alphavbeta6-positive than in alphavbeta6-negative tumours. In further studies with MCF10.DCIS.COM and MCF10A.CA1a breast carcinoma cell lines, which express alphavbeta6 endogenously, the radiopeptide achieved similar levels of tumour retention and permitted excellent discriminatory imaging of tumours. Thus, A20FMDV2 can be used for molecular-specific targeting of alphavbeta6 for imaging in vivo the often more aggressive, alphavbeta6-positive canc...Continue Reading
References
Apr 3, 1992·Cell·R O Hynes
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Mar 4, 1998·British Journal of Cancer·T MeyerI R Hart
Aug 26, 1998·Biochemical and Biophysical Research Communications·J NiuM Agrez
May 9, 2000·Journal of Virology·T JacksonA M King
Mar 23, 2001·Breast Cancer Research and Treatment·S J SantnerF R Miller
May 8, 2001·International Journal of Cancer. Journal International Du Cancer·G J ThomasP M Speight
Jul 10, 2001·The Journal of Investigative Dermatology·G J ThomasJ F Marshall
Oct 26, 2001·Biochemical and Biophysical Research Communications·H XueR Pytela
Dec 26, 2003·The American Journal of Pathology·Lari HäkkinenHannu Larjava
Aug 4, 2004·The Journal of Cell Biology·Sam M Janes, Fiona M Watt
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
Sep 28, 2005·The Journal of General Virology·Paul MonaghanTerry Jackson
Jan 6, 2006·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G J ThomasJ F Marshall
Mar 7, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jeremy K BrownDavid D S Collie
Nov 17, 2006·Cancer Research·Maria L NystromGareth J Thomas
Jan 4, 2007·The American Journal of Pathology·Kyungmin HahmShelia M Violette
Jan 25, 2007·The Journal of Biological Chemistry·Danielle DiCaraJohn F Marshall
Apr 3, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Martin GotthardtOtto Boerman
May 16, 2007·The Journal of Pathology·S HazelbagG J Fleuren
Jun 19, 2007·Cancer Research·Anissa N ElayadiKathlynn C Brown
Aug 19, 2007·Cancer Research·Sven H HausnerJulie L Sutcliffe
Nov 30, 2007·Journal of Virology·Danielle DicaraTerry Jackson
Jan 18, 2008·Cancer Research·Louise A Koopman Van AarsenShelia M Violette
Jul 29, 2008·Journal of Molecular Biology·Heide KogelbergKerry Chester
Jun 12, 2009·Journal of Comparative Pathology·V O'DonnellB Baxt
Citations
Sep 14, 2011·Cancer Immunology, Immunotherapy : CII·Shao-Bo YangPing-Chang Yang
Aug 21, 2013·PloS One·Y K Stella ManAhuva Nissim
May 14, 2014·PloS One·Seong Beom AhnMark S Baker
Mar 4, 2014·Biochemical and Biophysical Research Communications·Masashi UedaHideo Saji
Jan 23, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lina Y HuJulie L Sutcliffe
Jul 12, 2014·BioMed Research International·Roland HaubnerOlaf Prante
Apr 21, 2017·Scientific Reports·Simona CamoraniLaura Cerchia
Jan 4, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Mayca OnegaJan Passchier
Feb 28, 2014·The Journal of Pathology·Karwan A MoutasimGareth J Thomas
Mar 26, 2021·Frontiers in Oncology·Nattaporn PhanthapholPa-Thai Yenchitsomanus
Dec 28, 2018·ACS Nano·Da HuangMatteo Palma