Jan 7, 1976

High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA

Journal of Chromatography
D B LakingsJ E Mrochek


Methylated and major purine and pyrimidine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the hydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated sample of tRNA were hydrolyzed to the free bases with a trifluoroacetic acid-formic acid mixture of 200 degrees. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.

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Mentioned in this Paper

Transfer RNA
Malignant Neoplasms
Bos indicus
Hydrogen-Ion Concentration

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