High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to-macrophage transition.

Glycobiology
Hannes HinneburgMorten Thaysen-Andersen

Abstract

Protein glycosylation impacts the development and function of innate immune cells. The glycophenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described. Herein, label-free porous graphitised carbon-liquid chromatography-tandem mass spectrometry (PGC-LC-MS/MS) was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition. Primary blood-derived CD14+ monocytes were differentiated ex vivo in the absence of strong anti- and proinflammatory stimuli using a conventional 7-day granulocyte-macrophage colony-stimulating factor differentiation protocol with longitudinal sampling. Morphology and protein expression monitored by light microscopy and proteomics validated the maturation process. Glycomics demonstrated that monocytes and macrophages display similar N-glycome profiles, comprising predominantly paucimannosidic (Man1-3GlcNAc2Fuc0-1, 22.1-30.8%), oligomannosidic (Man5-9GlcNAc2, 29.8-35.7%) and α2,3/6-sialylated complex-type N-glycans with variable core fucosylation (27.6-39.1%). Glycopeptide analysis validated conjugation of these glycans to human proteins, while quantitative proteo...Continue Reading

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Citations

Jul 16, 2020·The Journal of Biological Chemistry·Vignesh VenkatakrishnanAnna Karlsson-Bengtsson
Nov 1, 2020·Molecular & Cellular Proteomics : MCP·Rebeca KawaharaGiuseppe Palmisano
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Ronnie BlazevBenjamin L Parker
Feb 5, 2021·Journal of Clinical Medicine·Sayantani ChatterjeeMorten Thaysen-Andersen
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Ronnie BlazevBenjamin L Parker
Mar 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Depeng YangYu Li
Aug 28, 2020·Molecular Aspects of Medicine·Julian UgonottiMorten Thaysen-Andersen

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