High-resolution low-dose scanning transmission electron microscopy.

Journal of Electron Microscopy
James P BubanHenning Stahlberg

Abstract

During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording with a conventional field-emission gun STEM, while maintaining the high-resolution capability of the instrument. Our findings show that a combination of reduced pixel dwell time and reduced gun current can achieve radiation doses comparable to low-dose TEM.

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Citations

Apr 13, 2013·Micron : the International Research and Review Journal for Microscopy·F KrumeichR A Wepf
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