High-resolution magic angle spinning (1) H NMR measurement of ligand concentration in solvent-saturated chromatographic beads

Magnetic Resonance in Chemistry : MRC
Fredrik Elwinger, István Furó

Abstract

A method based on (1) H high-resolution magic angle spinning NMR has been developed for measuring concentration accurately in heterogeneous materials like that of ligands in chromatography media. Ligand concentration is obtained by relating the peak integrals for a butyl ligand in the spectrum of a water-saturated chromatography medium to the integral of the added internal reference. The method is fast, with capacity of 10 min total sample preparation and analysis time per sample; precise, with a reproducibility expressed as 1.7% relative standard deviation; and accurate, as indicated by the excellent agreement of derived concentration with that obtained previously by (13) C single-pulse excitation MAS NMR. The effects of radiofrequency field inhomogeneity, spin rate, temperature increase due to spinning, and distribution and re-distribution of medium and reference solvent both inside the rotor during spinning and between bulk solvent and pore space are discussed in detail. © 2016 The Authors Magnetic Resonance in Chemistry published by John Wiley & Sons Ltd.

References

Sep 19, 1998·Journal of Magnetic Resonance·S Bourg, J M Nuzillard
Mar 18, 1999·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·K MillisS Singer
Mar 24, 2000·Journal of Magnetic Resonance·Y Q Song
Jun 27, 2000·Solid State Nuclear Magnetic Resonance·J Brus
Jan 10, 2001·Journal of Combinatorial Chemistry·R HanyD Obrecht
Aug 27, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jennifer L TaylorLeo L Cheng
Dec 4, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Chin-Lee WuLeo L Cheng
Sep 15, 2004·Magnetic Resonance in Chemistry : MRC·Sergey V DvinskikhDick Sandström
Oct 14, 2004·Analytical and Bioanalytical Chemistry·Laura H LucasCynthia K Larive
Feb 1, 2005·Journal of Natural Products·Guido F PauliDavid C Lankin
Oct 19, 2005·Solid State Nuclear Magnetic Resonance·Fabio Ziarelli, Stefano Caldarelli
May 11, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mark G SwansonJohn Kurhanewicz
Aug 21, 2007·Journal of Magnetic Resonance·Fabio ZiarelliStefano Caldarelli
Feb 24, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mark J AlbersJohn Kurhanewicz
Dec 2, 2009·Magnetic Resonance in Chemistry : MRC·Volker FriebolinKlaus Albert
Nov 1, 2013·Journal of Pharmaceutical and Biomedical Analysis·Katarzyna Paradowska, Iwona Wawer
Nov 7, 2014·Analytical Chemistry·Cynthia K LariveMeredith M Dinges
Jun 9, 2015·Magnetic Resonance in Chemistry : MRC·Fredrik ElwingerIstván Furó

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
NMR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Magnetic Resonance in Chemistry : MRC
Karol Jackowski, Włodzimierz Makulski
© 2022 Meta ULC. All rights reserved