High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish.

Communications Biology
Alexander HeckerStefan Schuster

Abstract

In non-mammalian vertebrates, some neurons can regenerate after spinal cord injury. One of these, the giant Mauthner (M-) neuron shows a uniquely direct link to a robust survival-critical escape behavior but appears to regenerate poorly. Here we use two-photon microscopy in parallel with behavioral assays in zebrafish to show that the M-axon can regenerate very rapidly and that the recovery of functionality lags by just days. However, we also find that the site of the injury is critical: While regeneration is poor both close and far from the soma, rapid regeneration and recovery of function occurs for injuries between 10% and 50% of total axon length. Our findings show that rapid regeneration and the recovery of function can be studied at remarkable temporal resolution after targeted injury of one single M-axon and that the decision between poor and rapid regeneration can be studied in this one axon.

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Citations

Jul 3, 2021·Cells·Vasiliki Tsata, Daniel Wehner
Jun 30, 2021·The Journal of Physiology·Gal HaspelJennifer R Morgan

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

R Studio
lme4
emmeans
Fiji
GraphPad
GraphPad Prism
R

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