High-resolution mass spectrometry of small molecules bound to membrane proteins.

Nature Methods
Joseph GaultCarol V Robinson

Abstract

Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

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Citations

May 21, 2016·Analytical Chemistry·Kin Kuan HoiMichael T Marty
Aug 3, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liliana Pedro, Ronald J Quinn
Aug 11, 2016·Biochemistry·Samuel Furse, David J Scott
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Dec 8, 2016·Journal of the American Society for Mass Spectrometry·Yang LiuArthur Laganowsky
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Aug 17, 2018·Proceedings of the National Academy of Sciences of the United States of America·Di WuCarol V Robinson
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Software Mentioned

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