High resolution melting curve assay for detecting rs12979860 IL28B polymorphisms involved in response of Iranian patients to chronic hepatitis C treatment

Asian Pacific Journal of Cancer Prevention : APJCP
Abolfazl FatehSeyed Hamid Reza Monavari

Abstract

A recent genome-wide association study (GWAS) on patients with chronic hepatitis C (CHC) treated with peginterferon and ribavirin (pegIFN-α/RBV) identified a single nucleotide polymorphism (SNP) on chromosome 19 (rs12979860) which was strongly associated with a sustained virological response (SVR). The aim of this study was twofold: to study the relationship between IL28B rs12979860 and sustained virological response (SVR) to pegIFN-α/RVB therapy among CHC patients and to detect the rs12979860 polymorphism by high resolution melting curve (HRM) assay as a simple, fast, sensitive, and inexpensive method. The study examined outcomes in 100 patients with chronic hepatitis C in 2 provinces of Iran from December 2011 to June 2013. Two methods were applied to detect IL28B polymorphisms: PCR-sequencing as a gold standard method and HRM as a simple, fast, sensitive, and inexpensive method. The frequencies of IL28B rs12979860 CC, CT, and TT alleles in chronic hepatitis C genotype 1a patients were 10% (10/100), 35% (35/100), and 6% (6/100) and in genotype 3a were 13% (13/100), 31% (31/100), and 5% (5/100), respectively. In genotype 3a infected patients, rs12979860 (CC and CT alleles) and in genotype 1a infected patients (CC allele) were ...Continue Reading

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Citations

Mar 24, 2018·Scientific Reports·Yazdan BijvandAbolfazl Fateh
Jul 26, 2019·Future Oncology·Mohammad FarahmandAhmad Tavakoli
May 6, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Amel G ElsheredyTamer Haydara
Sep 10, 2020·Current HIV Research·Mohsen MoghoofeiSeyed Hamidreza Monavari

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