High-resolution melting-curve (HRM) analysis for C. meleagridis identification in stool samples

Microbial Pathogenesis
Hanen ChelbiKarim Aoun

Abstract

Cryptosporidiosis represents a major public health problem. This infection, caused by a protozoan parasite of the genus Cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea. In the immunocompetent host, the typical watery diarrhea can be self-limiting. However, it is severe and chronic, in the immunocompromised host and may cause death. Cryptosporidium spp. are coccidians, which complete their life cycle in both humans and animals. The two species C. hominis and C. parvum are the major cause of human infection. Compared to studies on C. hominis and C. parvum, only a few studies have developed methods to identify C. meleagridis. To develop a new real time PCR-coupled High resolution melting assay allowing the detection for C. meleagridis, in addition of the other dominant species (C. hominis and C. parvum). The polymorphic sequence on the dihydrofolate reductase gene (DHFR) of three species was sequenced to design primers pair and establish a sensitive real-time PCR coupled to a high-resolution melting-curve (HRM) analysis method, allowing the detection of Cryptosporidium sp. and discrimination between three prevalent species in Tunisia. We analyzed a collection of 42 archived human isolates of the three...Continue Reading

Citations

Apr 14, 2021·International Journal for Parasitology·Jiayu LiLihua Xiao
May 28, 2021·European Journal of Protistology·Jennifer K O'LearyBrigid Lucey
May 17, 2021·Experimental Parasitology·Jennifer K O' LearyBrigid Lucey
Sep 3, 2021·Food and Waterborne Parasitology·Jennifer K O'LearyBrigid Lucey

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