High-resolution microbial community succession of microbially induced concrete corrosion in working sanitary manholes

PloS One
Alison L LingMark T Hernandez

Abstract

Microbially-induced concrete corrosion in headspaces threatens wastewater infrastructure worldwide. Models for predicting corrosion rates in sewer pipe networks rely largely on information from culture-based investigations. In this study, the succession of microbes associated with corroding concrete was characterized over a one-year monitoring campaign using rRNA sequence-based phylogenetic methods. New concrete specimens were exposed in two highly corrosive manholes (high concentrations of hydrogen sulfide and carbon dioxide gas) on the Colorado Front Range for up to a year. Community succession on corroding surfaces was assessed using Illumina MiSeq sequencing of 16S bacterial rRNA amplicons and Sanger sequencing of 16S universal rRNA clones. Microbial communities associated with corrosion fronts presented distinct succession patterns which converged to markedly low α-diversity levels (< 10 taxa) in conjunction with decreasing pH. The microbial community succession pattern observed in this study agreed with culture-based models that implicate acidophilic sulfur-oxidizer Acidithiobacillus spp. in advanced communities, with two notable exceptions. Early communities exposed to alkaline surface pH presented relatively high α-dive...Continue Reading

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Citations

Jul 9, 2016·Environmental Science & Technology·Guangming JiangPhilip L Bond
May 6, 2017·Frontiers in Microbiology·Xuan LiPhilip L Bond
Mar 16, 2017·Environmental Science & Technology·Natalie M HullNorman R Pace

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Datasets Mentioned

BETA
PRJNA201330
KF844359
KF845940

Methods Mentioned

BETA
amplicon
PCR
PCRs
electrophoresis
amplicon sequencing

Software Mentioned

MiSeq Control
phrap
Explicet
CrossMatch
R Statistics
SINA
Univec
NDS

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