High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording

Scientific Reports
Dong-Jie ZhaoLan Huang

Abstract

The limitations of conventional extracellular recording and intracellular recording make high-resolution multisite recording of plant bioelectrical activity in situ challenging. By combining a cooled charge-coupled device camera with a voltage-sensitive dye, we recorded the action potentials in the stem of Helianthus annuus and variation potentials at multiple sites simultaneously with high spatial resolution. The method of signal processing using coherence analysis was used to determine the synchronization of the selected signals. Our results provide direct visualization of the phloem, which is the distribution region of the electrical activities in the stem and leaf of H. annuus, and verify that the phloem is the main action potential transmission route in the stems of higher plants. Finally, the method of optical recording offers a unique opportunity to map the dynamic bioelectrical activity and provides an insight into the mechanisms of long-distance electrical signal transmission in higher plants.

References

Apr 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·Z KrasznaiL Trón
Apr 24, 1999·The Plant Cell·K J Oparka, R Turgeon
Jan 18, 2007·Planta·Hubert H Felle, Matthias R Zimmermann
Feb 1, 2007·Plant, Cell & Environment·Jörg Fromm, Silke Lautner
Jul 7, 2007·Journal of Experimental Botany·Alexandra C U FurchAart J E van Bel
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·E MasiS Mancuso
Jan 1, 2006·Plant Signaling & Behavior·Rainer StahlbergElizabeth Van Volkenburgh
Mar 2, 2010·FEBS Letters·Stanislav IsayenkovFrans J M Maathuis
Apr 8, 2010·Comptes rendus biologies·Sylvie Dinant, Rémi Lemoine
Jul 16, 2010·Journal of Plant Physiology·Patricio Oyarce, Luis Gurovich
Nov 30, 2010·Journal of Plant Physiology·Patrick FavreRobert Degli Agosti
May 12, 2011·Journal of Cell Science·Chiara CalliesHans Oberleithner
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Aart J E van BelJens B Hafke
Sep 29, 2012·Plant Science : an International Journal of Experimental Plant Biology·Simon A ZebeloMassimo E Maffei
Nov 13, 2012·Journal of Molecular and Cellular Cardiology·José M Di DiegoCharles Antzelevitch
Feb 21, 2013·Journal of Plant Physiology·Alexander G VolkovVladislav S Markin
Jun 12, 2013·Journal of Plant Physiology·Alexander G VolkovVladislav S Markin
Jul 13, 2013·Frontiers in Plant Science·Jörg FrommSilke Lautner
Aug 24, 2013·Nature·Seyed A R MousaviEdward E Farmer
Jan 30, 2014·Journal of Biomedical Optics·M Caroline MüllenbroichKeith Mathieson
Feb 1, 2014·Journal of Experimental Botany·Aart J E van BelJens B Hafke
Mar 13, 2014·Archives of Environmental Contamination and Toxicology·Felipe TuccaRicardo Barra
Mar 19, 2014·Plant, Cell & Environment·Vladimir SukhovVladimir Vodeneev
Jan 16, 2015·Scientific Reports·Elisa MasiStefano Mancuso
Jan 30, 2015·Journal of the Royal Society, Interface·Shre Kumar ChatterjeeAndrea Vitaletti

❮ Previous
Next ❯

Citations

Feb 18, 2016·Trends in Plant Science·Rainer HedrichIngo Dreyer
May 8, 2016·Photosynthesis Research·Vladimir Sukhov
Sep 7, 2017·Plant, Cell & Environment·Paco CalvoAnthony Trewavas
Jul 18, 2017·The Journal of Membrane Biology·Ekaterina SukhovaVladimir Sukhov
Apr 23, 2021·Journal of Plant Physiology·Jin-Hai LiLan Huang
Jun 1, 2021·The Plant Journal : for Cell and Molecular Biology·Yosef Fichman, Ron Mittler
Jun 15, 2021·Plant Signaling & Behavior·Sergio Miguel-Tomé, Rodolfo R Llinás

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence collection

Software Mentioned

Tsview7
DiBAC
Matlab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.