High-resolution paramagnetically enhanced solid-state NMR spectroscopy of membrane proteins at fast magic angle spinning

Journal of Biomolecular NMR
Meaghan E WardVladimir Ladizhansky

Abstract

Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the study of membrane proteins in membrane mimetic and native membrane environments. These experiments often suffer from low sensitivity, due in part to the long recycle delays required for magnetization and probe recovery, as well as detection of low gamma nuclei. In ultrafast MAS experiments sensitivity can be enhanced through the use of low power sequences combined with paramagnetically enhanced relaxation times to reduce recycle delays, as well as proton detected experiments. In this work we investigate the sensitivity of (13)C and (1)H detected experiments applied to 27 kDa membrane proteins reconstituted in lipids and packed in small 1.3 mm MAS NMR rotors. We demonstrate that spin diffusion is sufficient to uniformly distribute paramagnetic relaxation enhancement provided by either covalently bound or dissolved CuEDTA over 7TM alpha helical membrane proteins. Using paramagnetic enhancement and low power decoupling in carbon detected experiments we can recycle experiments ~13 times faster than under traditional conditions. However, due to the small sample volume the overall sensitivity per unit time is still lower than that seen in the 3.2 mm prob...Continue Reading

References

Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 11, 2002·Journal of Molecular Biology·Thomas FriedrichErnst Bamberg
Mar 8, 2003·Molecular Microbiology·Kwang-Hwan JungJohn L Spudich
Jun 18, 2003·Journal of Magnetic Resonance·Corey R Morcombe, Kurt W Zilm
Apr 15, 2004·Journal of the American Chemical Society·Matthias ErnstBeat H Meier
Mar 15, 2006·Journal of Molecular Biology·Lichi ShiLeonid S Brown
Aug 30, 2007·Journal of the American Chemical Society·Donghua H ZhouChad M Rienstra
Oct 10, 2007·Journal of Magnetic Resonance·Rasmus LinserBernd Reif
Dec 25, 2007·Magnetic Resonance in Chemistry : MRC·Mrignayani KotechaYoshitaka Ishii
Feb 10, 2009·Nature Methods·Nalinda P WickramasingheYoshitaka Ishii
Feb 27, 2009·Journal of Molecular Biology·Lichi ShiVladimir Ladizhansky
Feb 28, 2009·Annual Review of Biophysics·Ann McDermott
Apr 24, 2009·Solid State Nuclear Magnetic Resonance·Ayyalusamy Ramamoorthy
Jul 16, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Vinesh VijayanAdam Lange
Oct 6, 2009·Biochimica Et Biophysica Acta·Lichi ShiVladimir Ladizhansky
May 4, 2010·Journal of the American Chemical Society·Kazutoshi YamamotoAyyalusamy Ramamoorthy
May 29, 2010·Journal of the American Chemical Society·Józef R LewandowskiLyndon Emsley
Jun 30, 2010·Journal of the American Chemical Society·Philippe S NadaudChristopher P Jaroniec
Aug 18, 2010·Doklady. Biochemistry and Biophysics·T A Egorova-ZachernyukV I Shvets
Oct 14, 2010·Angewandte Chemie·Marie RenaultMarc Baldus
Oct 16, 2010·Applied Microbiology and Biotechnology·Tatiana A Egorova-ZachernyukWillem J Degrip
Oct 28, 2010·Journal of the American Chemical Society·Paul SchandaMatthias Ernst
Feb 4, 2011·Angewandte Chemie·Lichi ShiVladimir Ladizhansky
Aug 16, 2011·The Journal of Physical Chemistry Letters·Ming TangChad M Rienstra
Sep 17, 2011·Journal of the American Chemical Society·Meaghan E WardVladimir Ladizhansky
Apr 3, 2012·Solid State Nuclear Magnetic Resonance·Christopher P Jaroniec
Apr 24, 2012·Nature Chemistry·Ishita SenguptaChristopher P Jaroniec
Oct 4, 2012·Journal of the American Chemical Society·Shenlin WangVladimir Ladizhansky
Oct 23, 2012·Nature·Sang Ho ParkStanley J Opella
Nov 13, 2012·Nature Methods·Shakeel Ahmad ShahidDirk Linke

❮ Previous
Next ❯

Citations

Dec 3, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Shenlin Wang, Vladimir Ladizhansky
Nov 11, 2014·Journal of the American Chemical Society·Jonathan M LamleyJózef R Lewandowski
Mar 24, 2015·Journal of Magnetic Resonance·Christopher P Jaroniec
Feb 11, 2015·Journal of Biomolecular NMR·Andrew J NieuwkoopHartmut Oschkinat
Jun 1, 2017·Journal of Biomolecular NMR·Chang LiuShenlin Wang
Sep 25, 2016·Biological Chemistry·Susann ZilkenatSamuel Wagner
Dec 11, 2017·Journal of Magnetic Resonance·Yoshitaka IshiiTakayuki Kamihara
Oct 28, 2019·Journal of Chemical Theory and Computation·Malte SiemersAna-Nicoleta Bondar
Jun 15, 2017·Journal of the American Chemical Society·Daryl GoodVladimir Ladizhansky
Nov 6, 2020·The Journal of Physical Chemistry Letters·Yimin MiaoMing Tang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.