PMID: 7016174Apr 14, 1981Paper

High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals

Biochemistry
D G GorensteinB A Luxon

Abstract

The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site of phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal in experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P..J.M., Swarthof T., & Hilbers, C.W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.

References

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Citations

Nov 28, 1984·Biochimica Et Biophysica Acta·M P SchweizerG B Chheda
Aug 1, 1987·Quarterly Reviews of Biophysics·D J PatelD Hare
Feb 17, 1986·European Journal of Biochemistry·E I Hyde
Dec 1, 1988·European Journal of Biochemistry·F J Van de Ven, C W Hilbers
Oct 30, 2007·FEBS Letters·Elisabetta Viani Puglisi, Joseph D Puglisi
Nov 18, 2004·Journal of Magnetic Resonance·Cheuk Nang Ho, Sik Lok Lam
Jul 30, 1985·Biochemistry·E I Hyde, B R Reid

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