High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Miconia albicans

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Rita de Cássia Lemos LimaDan Staerk

Abstract

Protein tyrosine phosphatase 1B (PTP1B) is an intracellular enzyme responsible for deactivation of the insulin receptor, and consequently acts as a negative regulator of insulin signal transduction. In recent years, PTP1B has become an important target for controlling insulin resistance and type 2 diabetes. In the present study, the ethyl acetate extract of leaves of Miconia albicans (IC50 = 4.92 µg/mL) was assessed by high-resolution PTP1B inhibition profiling combined with HPLC-HRMS-SPE-NMR for identification of antidiabetic compounds. This disclosed eleven PTP1B inhibitors, including five polyphenolics: 1-O-(E)-caffeoyl-4,6-di-O-galloyl-β-d-glucopyranose (2), myricetin 3-O-α-l-rhamnopyranoside (3), quercetin 3-O-(2″-galloyl)-α-l-rhamnopyranoside (5), mearnsetin 3-O-α-l-rhamnopyranoside (6), and kaempferol 3-O-α-l-arabinopyranoside (8) as well as eight triterpenoids: maslinic acid (13), 3-epi-sumaresinolic acid (14), sumaresinolic acid (15), 3-O-cis-p-coumaroyl maslinic acid (16), 3-O-trans-p-coumaroyl maslinic acid (17), 3-O-trans-p-coumaroyl 2α-hydroxydulcioic acid (18), oleanolic acid (19), and ursolic acid (20). These results support the use of M. albicans as a traditional medicine with antidiabetic properties and its pot...Continue Reading

References

May 9, 2000·Memórias do Instituto Oswaldo Cruz·T M AlvesC L Zani
Jul 21, 2000·The Journal of Clinical Investigation·G I Shulman
Jul 18, 2001·Chemical & Pharmaceutical Bulletin·Z H JiangI Kouno
Dec 12, 2001·Phytochemistry·I I MahmoudA M Hassan
Jan 31, 2003·Expert Opinion on Investigational Drugs·Zhong-Yin Zhang, Seung-Yub Lee
Jun 13, 2003·Planta medica·Wilson Roberto CunhaSérgio Albuquerque
Jun 10, 2004·Cell·Andres AlonsoTomas Mustelin
Jun 29, 2004·Diabetes Care·George AlbertiUNKNOWN Consensus Workshop Group
May 1, 2007·Drug Discovery Today·Sheng Zhang, Zhong-Yin Zhang
Apr 14, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Juliana Mara SerpeloniIlce Mara de Syllos Cólus
Apr 2, 2011·European Journal of Medicinal Chemistry·Juan José Ramírez-EspinosaSamuel Estrada-Soto
Oct 11, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Eisuke ShibataTomoyuki Nishizaki
Apr 25, 2016·Archives of Pharmacal Research·Kyeong Wan WooKang Ro Lee
Aug 2, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·David Mizael Ortíz-MartinezCatalina Leos-Rivas
Mar 11, 2017·Chemistry & Biodiversity·Alessandro VendittiArmandodoriano Bianco
Jul 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Binh Thi Dieu TrinhDan Staerk
Aug 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kai-Kai GongGuo-Qiang Li

❮ Previous
Next ❯

Citations

Jul 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stavros BeteinakisMaria Halabalaki

❮ Previous
Next ❯

Datasets Mentioned

BETA
UFG
7163

Methods Mentioned

BETA
nuclear magnetic resonance
NMR
Assay

Software Mentioned

Prep Gilson ST
GraFit
Agilent ChemStation
Excel
Hystar

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.