High-resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Philipp E BayerDavid Edwards

Abstract

We characterise the distribution of crossover and non-crossover recombination in Brassica napus and Cicer arietinum using a low-coverage genotyping by sequencing pipeline SkimGBS. The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.

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Citations

Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Rajeev K Varshney
Mar 2, 2016·Current Opinion in Plant Biology·Jacqueline Batley, David Edwards
May 21, 2016·Frontiers in Plant Science·Manish K PandeyRajeev K Varshney
Nov 23, 2017·Frontiers in Plant Science·Ting Xiang NeikJacqueline Batley
Apr 14, 2017·Plant Biotechnology Journal·Philipp E BayerDavid Edwards
Dec 19, 2017·Frontiers in Plant Science·Haiying GuanQingchun Pan
Oct 19, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Aria DolatabadianJacqueline Batley
May 11, 2019·G3 : Genes - Genomes - Genetics·Mary M HappDavid L Hyten
Sep 4, 2019·Nature Genetics·Jonathan KreplakJudith Burstin
Feb 11, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ghader Mirzaghaderi, Annaliese S Mason
Dec 15, 2018·The Plant Journal : for Cell and Molecular Biology·Armin SchebenDavid Edwards
Dec 26, 2018·Frontiers in Plant Science·M Michelle MalmbergNoel O I Cogan
Dec 21, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kai Peter Voss-FelsBen John Hayes
Feb 7, 2017·Frontiers in Plant Science·Katarzyna GacekJacqueline Batley
Sep 8, 2019·Plants·Agnieszka TomkowiakPrzemysław Łukasz Kowalczewski
Dec 24, 2018·Trends in Plant Science·Khanh Le NguyenPascal Gantet
Oct 15, 2021·Genome Génome / Conseil National De Recherches Canada·Guoliang LiRifei Sun

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