High resolution structural studies of Cro repressor protein and implications for DNA recognition

Journal of Biomolecular Structure & Dynamics
D H OhlendorfB W Matthews

Abstract

Cro repressor is a small dimeric protein that binds to specific sites on the DNA of bacteriophage lambda. The structure of Cro has been determined and suggests that the protein binds to its sequence-specific sites with a pair of two-fold related alpha-helices of the protein located within successive major grooves of the DNA. From the known three-dimensional structure of the repressor, model building and energy refinement have been used to develop a detailed model for the presumed complex between Cro and DNA. Recognition of specific DNA binding sites appears to occur via multiple hydrogen bonds between amino acid side chains of the protein and base pair atoms exposed within the major groove of DNA. The Cro:DNA model is consistent with the calculated electrostatic potential energy surface of the protein. From a series of amino acid sequence and gene sequence comparisons, it appears that a number of other DNA-binding proteins have an alpha-helical DNA-binding region similar to that seen in Cro. The apparent sequence homology includes not only DNA-binding proteins from different bacteriophages, but also gene-regulatory proteins from bacteria and yeast. It has also been found that the conformations of part of the presumed DNA-bindin...Continue Reading

References

Aug 16, 1979·Nature·P J ArtymiukM J Sternberg
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T A SteitzB W Matthews
Feb 1, 1983·Cell·A HochschildM Ptashne
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M H HechtR T Sauer
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·H C NelsonR T Sauer
Jan 1, 1983·Annual Review of Biophysics and Bioengineering·D H Ohlendorf, B W Matthews
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·T A SteitzJ B Matthew
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·B W MatthewsY Takeda
Aug 25, 1982·Nucleic Acids Research·I T WeberT A Steitz
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B W MatthewsY Takeda
Sep 28, 1982·Biochemistry·J B Matthew, F M Richards
Mar 22, 2008·Science·Shintaro IshiwataYoshinori Tokura

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Citations

Feb 1, 1997·Current Opinion in Structural Biology·M A KercherM Lewis
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I T Weber, T A Steitz
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K W PlaxcoW A Goddard
Nov 26, 1984·Nucleic Acids Research·I T Weber, T A Steitz
Jun 1, 1986·Journal of Biomolecular Structure & Dynamics·M Cygler, W F Anderson
Jan 1, 1985·CRC Critical Reviews in Biochemistry·J B MatthewS J Shire
Dec 22, 1992·Biochemistry·Y V GrikoP L Privalov
Jan 5, 1991·Journal of Molecular Biology·K YanagiR E Dickerson
Dec 1, 1984·Biopolymers·J B Matthew, F M Richards
Mar 5, 1986·Journal of Molecular Biology·R G BrennanB W Matthews
Aug 13, 2015·The Journal of Physical Chemistry Letters·John Yao, Jin Wang

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