High-Resolution, Three-Dimensional Reconstruction of the Outflow Tract Demonstrates Segmental Differences in Cleared Eyes

Investigative Ophthalmology & Visual Science
Susannah WaxmanNils A Loewen

Abstract

The rate of conventional aqueous humor outflow is the highest nasally. We hypothesized that this is reflected in regionally different outflow structures and analyzed the entire limbus by high-resolution, full-thickness ribbon-scanning confocal microscopy (RSCM). We perfused pig eyes by anterior chamber cannulation with eight lectin-fluorophore conjugates, followed by optical clearance with benzyl alcohol benzyl benzoate (BABB). RSCM and advanced analysis software (Imaris) were used to reconstruct a three-dimensional (3D), whole-specimen rendering of the perilimbal outflow structures. We performed morphometric analyses of the outflow tract from the level of the trabecular meshwork (TM) to the scleral vascular plexus (SVP). Except for pigmented structures, BABB cleared the entire eye. Rhodamine-conjugated Glycine max agglutinin (soybean [SBA]) labeled the outflow tract evenly and retained fluorescence for months. RSCM produced terabyte-sized files allowing for in silico dissection of outflow tract vessels at a high resolution and in 3D. Networks of interconnected lumens were traced from the TM to downstream drainage structures. The collector channel (CC) volumes were 10 times smaller than the receiving SVP vessels, the largest of...Continue Reading

References

Jun 1, 1992·Experimental Eye Research·O Mäepea, A Bill
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·K M Meek, N J Fullwood
Jun 1, 1963·Archives of Ophthalmology·W M GRANT
Oct 2, 2003·Biology of the Cell·W B Amos, J G White
Aug 12, 2008·Ophthalmology·Boel BengtssonUNKNOWN EMGT Group
May 5, 2009·Biochimica Et Biophysica Acta·Josephine V Glenn, Alan W Stitt
Dec 4, 2009·Methods in Molecular Biology·Benedikt W Graf, Stephen A Boppart
Dec 1, 2005·BioTechniques·Claudette M St CroixSimon C Watkins
Mar 9, 2010·Investigative Ophthalmology & Visual Science·Yolanda Fernández-BarrientosJulian García Sánchez
May 7, 2010·Nature·Suphansa SawamiphakAmparo Acker-Palmer
May 8, 2010·Experimental Eye Research·E L van der Merwe, S H Kidson
Jun 19, 2010·Nature Chemical Biology·Andreas Schmid, Lars M Blank
Jan 13, 2012·PloS One·Sujiv VurgeseJost B Jonas
Apr 6, 2012·PloS One·Klaus BeckerHans-Ulrich Dodt
Apr 14, 2012·Investigative Ophthalmology & Visual Science·Andrew W FrancisJoel S Schuman
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Feb 14, 2013·Investigative Ophthalmology & Visual Science·Cathy NguyenHarry A Quigley
Apr 19, 2013·Nature Reviews. Genetics·Bonnie BergerMona Singh
Nov 19, 2013·Investigative Ophthalmology & Visual Science·Cherie B NauArthur J Sit
Jan 25, 2014·Proteomics. Clinical Applications·Adam E SienkiewiczSanjoy K Bhattacharya
Jul 26, 2014·The Journal of Clinical Investigation·Dae-Young ParkGou Young Koh
Jul 26, 2014·The Journal of Clinical Investigation·Aleksanteri AspelundKari Alitalo
Aug 2, 2014·European Journal of Anaesthesiology·Daniel ChappellBernhard F Becker
Aug 30, 2014·The British Journal of Ophthalmology·I I BusselUNKNOWN Trabectome Study Group
Sep 15, 2014·Experimental Eye Research·Chen-Yuan Charlie YangHaiyan Gong
Oct 7, 2014·Experimental Eye Research·Elizabeth C KimballHarry A Quigley
Oct 23, 2014·The British Journal of Ophthalmology·I I BusselUNKNOWN Trabectome Study Group
Jul 18, 2015·Cell·Douglas S Richardson, Jeff W Lichtman
Aug 15, 2015·PloS One·April K ScruggsNatalie N Bauer
Oct 9, 2015·Nature Protocols·Etsuo A SusakiHiroki R Ueda
Jan 16, 2016·Microscopy Research and Technique·Hanae KataokaTakehiko Iijima
Mar 24, 2016·Investigative Ophthalmology & Visual Science·Ralitsa T LoewenNils A Loewen
May 5, 2016·Progress in Histochemistry and Cytochemistry·Adriano AzaripourCornelis J F Van Noorden
May 27, 2016·Investigative Ophthalmology & Visual Science·Ralitsa T LoewenNils A Loewen
Oct 30, 2016·Journal of Clinical & Experimental Ophthalmology·Alex S HuangRobert N Weinreb

❮ Previous
Next ❯

Citations

Apr 5, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ying HongNils A Loewen
Sep 26, 2019·Communications Biology·Sayan GhoshDebasish Sinha
Apr 30, 2019·Journal of Cataract and Refractive Surgery·Hamed EsfandiariNils A Loewen
Aug 22, 2020·Translational Vision Science & Technology·Chen XinNingli Wang
Sep 28, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Hamed EsfandiariNils A Loewen
Apr 4, 2020·Scientific Reports·Fiona McDonnellW Daniel Stamer
Sep 17, 2020·Translational Vision Science & Technology·Jessica B BurnChris G Pirie
Oct 17, 2019·Translational Vision Science & Technology·Kevin C SnyderGillian J McLellan
Sep 6, 2020·Journal of Glaucoma·Kae SugiharaYuki Morizane
Jan 12, 2020·Communications Biology·Sayan GhoshDebasish Sinha
Nov 21, 2020·Progress in Retinal and Eye Research·Murray JohnstoneRuikang K Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
light scattering
glycosylation
dissecting
electron microscopy
light-scattering

Software Mentioned

FocusClear
Imaris
ImageJ
iDISCO
RSCM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.