High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression.

Blood
Marianna Kulka, D D Metcalfe

Abstract

T-helper 1 (TH1) (interferon-gamma [IFN-gamma]) and TH2 (interleukin-4 [IL-4] and IL-5) cytokines have been variably reported to alter human mast cell numbers in complex culture systems. The effects of these cytokines on the kinetics of cell division and cell death are unknown, and their effect on mast cell behavior is relevant to anticipate the consequences of in vivo strategies that alter cytokine levels. To determine the effect of these cytokines on stem cell factor (SCF)-dependent human mast cell production, we used high-resolution tracking of cell division and correlated the results with cell apoptosis, expression of Kit, and mast cell degranulation. When IFN-gamma, IL-5, or IL-4 was administered over 8 weeks, we found each cytokine decreased the mast number through a different mechanism. IFN-gamma inhibited early progenitor cell division, IL-4 down-regulated early Kit expression, and IL-5 blocked later cell division. Further, IL-4 and IFN-gamma had the greatest suppressive effect on degranulation and FcepsilonRI expression. When these cytokines were administered to mature mast cells, IFN-gamma and IL-5 had no effect on degranulation and cell division, but IL-4 induced division and potentiated FcepsilonRI-mediated degranul...Continue Reading

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Citations

Jul 5, 2011·Glycobiology·Simon J NorthStuart M Haslam
Aug 8, 2008·Blood·Dean D Metcalfe
Jul 25, 2009·Journal of Leukocyte Biology·Marianna KulkaDean D Metcalfe
Jun 18, 2009·Seminars in Immunopathology·Stephan C Bischoff
Nov 17, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Peter Bradding, Greer Arthur
Jul 19, 2011·The Journal of Allergy and Clinical Immunology·Todd M WilsonAmy D Klion
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Jun 5, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shuhei YoshidaDetlef Schuppan
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N A MolfinoG P Geba
Oct 10, 2006·Veterinary Immunology and Immunopathology·Tzu-Yin Lin, Cheryl A London
Jun 20, 2006·Veterinary Immunology and Immunopathology·Tzu-yin LinCheryl A London
Sep 21, 2017·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali Komi, Korneel Grauwet
Aug 29, 2017·Cell Death Discovery·Tarek HazzanMagda Babina
Mar 23, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew R MaceyJohn J Ryan
Aug 19, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Priyanka PundirMarianna Kulka
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nataliya M Kushnir-SukhovDean D Metcalfe
Feb 13, 2018·Immunological Reviews·Kaori MukaiStephen J Galli
Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Meredith N Mann-ChandlerJohn J Ryan
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yongfeng JiangJoshua A Boyce

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis