High resolution two-dimensional electrophoretic analysis of urinary proteins of patients with prostatic cancer

Electrophoresis
P K Grover, Martin I Resnick

Abstract

In an attempt to identify marker(s) for prostatic cancer, proteins in urines of normal and prostatectomized males and in men with cancerous prostate were analyzed. Only urines collected with protease inhibitors were examined. Two-dimensional (2-D) gel electrophoresis was used for high resolution separation of proteins and the electrophoretograms were either developed by double stain or the proteins were electrophoretically transferred onto nitrocellulose for immunological identification. The pool of each group exhibited similar relative positions of major protein spots. The study of normal and prostatectomized men identified two proteins denoted as A (36 kDa, pI 6-6.5) and B (23 kDa, pI 6.6), which were undetectable in the latter group. A visual comparison of the patterns of normals and patients with cancerous prostate revealed that both these proteins were undetectable in urines of men with malignant prostatic carcinoma (PCA) and benign prostatic hyperplasia (BPH) and hence may be useful in identifying prostatic carcinoma. Also, while protein C 43.5 kDA, pI 6-6.6) was discerned in normals its abundance, along with those of proteins D (40 kDa, pI 6-6.4) and E (26.5 kDa, pI 6-6.7), appeared to be higher in BPA than in PCA. Prote...Continue Reading

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Citations

Aug 16, 2005·American Journal of Respiratory and Critical Care Medicine·Giovanni CandianoOlga Zegarra-Moran
Jun 29, 2013·Seminars in Oncology·Tapan BhavsarRuth Birbe
Mar 31, 2009·Critical Reviews in Oncology/hematology·Jingjing YouYong Li
Jul 10, 2008·Proteomics. Clinical Applications·Joshua J CoonPhilippe Schmitt-Kopplin
Sep 1, 2007·Proteomics. Clinical Applications·Magnus HellströmLars Egevad
Nov 11, 2005·Proteomics·Wei SunYouhe Gao
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Apr 23, 2003·Acta Biologica Hungarica·A MádiL Fésüs
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Sep 13, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·M R DownesS R Pennington
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Steven Skates, Othon Iliopoulos
Mar 20, 2003·Journal of Proteome Research·James X PangGregory J Opitek

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