High-Resolution Whole-Genome Analysis of Sister-Chromatid Contacts

Molecular Cell
Elena EspinosaFrançois-Xavier Barre

Abstract

Sister-chromatid cohesion describes the orderly association of newly replicated DNA molecules behind replication forks. It plays an essential role in the maintenance and faithful transmission of genetic information. Cohesion is created by DNA topological links and proteinaceous bridges, whose formation and deposition could be potentially affected by many processes. Current knowledge on cohesion has been mainly gained by fluorescence microscopy observation. However, the resolution limit of microscopy and the restricted number of genomic positions that can be simultaneously visualized considerably hampered progress. Here, we present a high-throughput methodology to monitor sister-chromatid contacts (Hi-SC2). Using the multi-chromosomal Vibrio cholerae bacterium as a model, we show that Hi-SC2 permits to monitor local variations in sister-chromatid cohesion at a high resolution over a whole genome.

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Citations

Sep 25, 2020·Nature Methods·Marlies E OomenJob Dekker
Dec 31, 2020·STAR Protocols·Elena EspinosaFrançois-Xavier Barre
Dec 24, 2020·Applied and Environmental Microbiology·Elena EspinosaElisa Galli
Aug 4, 2021·Annual Review of Microbiology·Virginia S LioyFrédéric Boccard
Dec 9, 2020··Elena EspinosaYoshiharu Yamaichi

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