High resolution X-ray structure of galactose mutarotase from Lactococcus lactis.

The Journal of Biological Chemistry
James B Thoden, Hazel M Holden

Abstract

Galactose mutarotase plays a key role in normal galactose metabolism by catalyzing the interconversion of beta-D-galactose and alpha-D-galactose. Here we describe the three-dimensional architecture of galactose mutarotase from Lactococcus lactis determined to 1.9-A resolution. Each subunit of the dimeric enzyme displays a distinctive beta-sandwich motif. This tertiary structural element was first identified in beta-galactosidase and subsequently observed in copper amine oxidase, hyaluronate lyase, chondroitinase, and maltose phosphorylase. Two cis-peptides are found in each subunit, namely Pro(67) and Lys(136). The active site is positioned in a rather open cleft, and the electron density corresponding to the bound galactose unequivocally demonstrates that both anomers of the substrate are present in the crystalline enzyme. Those residues responsible for anchoring the sugar to the protein include Arg(71), His(96), His(170), Asp(243), and Glu(304). Both His(96) and His(170) are strictly conserved among mutarotase amino acid sequences determined thus far. The imidazole nitrogens of these residues are located within hydrogen bonding distance to the C-5 oxygen of galactose. Strikingly, the carboxylate group of Glu(304) is situated ...Continue Reading

References

Dec 2, 1994·Journal of Molecular Biology·G G BouffardS L Adhya
Jun 30, 1994·Nature·R H JacobsonB W Matthews
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Jan 1, 1996·Biotechnology & Genetic Engineering Reviews·R A Prade
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·K Cowtan, P Main
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Apr 3, 2001·Applied and Environmental Microbiology·K A ErlandsonC A Batt

❮ Previous
Next ❯

Citations

May 5, 2005·Experimental Parasitology·Eduardo VillaloboDavid Mirelman
Mar 14, 2003·Journal of Molecular Biology·Pirkko HeikinheimoEdward Hough
Feb 7, 2004·European Journal of Biochemistry·Siddhartha MajumdarAmar Bhaduri
Dec 25, 2007·Journal of Bacteriology·Jason S RichardsonIvan J Oresnik
Jun 16, 2009·Journal of Molecular Biology·Kwang-Hoon LeeByung-Ha Oh
Apr 30, 2003·Protein Science : a Publication of the Protein Society·James B ThodenHazel M Holden
May 7, 2005·Journal of Molecular Biology·Kyoung-Seok RyuByong-Seok Choi
Jul 22, 2006·The Journal of Biological Chemistry·Marc GrailleHerman van Tilbeurgh
Aug 19, 2003·The Journal of Biological Chemistry·Hazel M HoldenJames B Thoden
Apr 13, 2004·Protein Science : a Publication of the Protein Society·Liliane AssairiAnne-Marie Gilles
May 20, 2003·FEBS Letters·David J Timson, Richard J Reece
Dec 21, 2007·The Journal of Biological Chemistry·David C Oliver, Mark Paetzel
Mar 30, 2005·The Journal of Biological Chemistry·James B Thoden, Hazel M Holden
Jun 11, 2003·The Journal of Biological Chemistry·James B Thoden, Hazel M Holden
Dec 23, 2003·European Journal of Biochemistry·Amrita Brahma, Debasish Bhattacharyya
Sep 10, 2002·The Journal of Biological Chemistry·James B ThodenHazel M Holden
Mar 18, 2004·The Journal of Biological Chemistry·James B ThodenHazel M Holden
Apr 26, 2021·The Journal of Biological Chemistry·Takfarinas KentacheEmile Van Schaftingen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.