High-risk human papillomavirus (hrHPV) E6/E7 mRNA testing by PreTect HPV-Proofer for detection of cervical high-grade intraepithelial neoplasia and cancer among hrHPV DNA-positive women with normal cytology.

Journal of Clinical Microbiology
D C RijkaartC J L M Meijer

Abstract

Our aim was to investigate whether high-risk HPV (hrHPV) mRNA detection by PreTect HPV-Proofer can be used to stratify hrHPV DNA-positive women of different cytology classes for risk of high-grade cervical intraepithelial neoplasia or worse (cervical precancer or cancer, i.e., cervical intraepithelial neoplasia grade 2 or higher [≥ CIN2]). A total of 375 women participating in population-based screening, with a GP5+/6+-PCR hrHPV DNA-positive cervical scrape with normal cytology (n = 202), borderline or mild dyskaryosis (BMD) (n = 88), or moderate dyskaryosis or worse (>BMD) (n = 85), were enrolled. Cervical scrapes were additionally subjected to HPV16/18/31/33/45 E6/E7 mRNA analysis by PreTect HPV-Proofer (mRNA test). Referral and follow-up policies were based on cytology, hrHPV DNA, and mRNA testing. The primary study endpoint was the number of ≥CIN2 detected within 3 years of follow-up. The mRNA positivity increased with the severity of cytological abnormality, ranging from 32% (64/202) in hrHPV DNA-positive women with normal cytology to 47% (41/88) in BMD and 68% (58/85) in >BMD groups (P < 0.01). Women with ≥ CIN2 were more likely to test positive by mRNA test (63%) than women without evidence of ≥ CIN2 (32%; P < 0.01). A p...Continue Reading

References

Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
May 4, 2000·Journal of the National Cancer Institute·H zur Hausen
Mar 7, 2002·Journal of Clinical Microbiology·Adriaan J C van den BrulePeter J F Snijders
Mar 29, 2002·International Journal of Cancer. Journal International Du Cancer·Mark van DuinChris J L M Meijer
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Jan 13, 2005·International Journal of Cancer. Journal International Du Cancer·Tor MoldenBjørn Hagmar
Mar 1, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Tor MoldenBjørn Hagmar
Feb 18, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Nabiha Saadi Abrahão TahaGerson Botacini Dôres
Mar 30, 2006·International Journal of Cancer. Journal International Du Cancer·Peter J F SnijdersChris J L M Meijer
Apr 6, 2006·International Journal of Cancer. Journal International Du Cancer·Jack CuzickThomas Iftner
Jul 13, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Johannes BerkhofChris J L M Meijer
Oct 2, 2007·American Journal of Obstetrics and Gynecology·Philip E CastleMark Schiffman
Oct 19, 2007·The New England Journal of Medicine·Marie-Hélène MayrandUNKNOWN Canadian Cervical Cancer Screening Trial Study Group
Oct 19, 2007·The New England Journal of Medicine·Pontus NauclerJoakim Dillner
Feb 2, 2008·International Journal of Cancer. Journal International Du Cancer·Jack CuzickWilliam P Soutter
Mar 28, 2008·Journal of the National Cancer Institute·Guglielmo RoncoUNKNOWN New Technologies for Cervical Cancer Screening Working Group
Sep 12, 2008·The Lancet Oncology·Francesca CarozziUNKNOWN New Technologies for Cervival Cancer Screening (NTCC) Working Group
Jan 15, 2009·Journal of the National Cancer Institute·Pontus NauclerJoakim Dillner
Jan 22, 2010·The Lancet Oncology·Guglielmo RoncoUNKNOWN New Technologies for Cervical Cancer screening (NTCC) Working Group
Mar 11, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Albertus T HesselinkPeter J F Snijders
Mar 15, 2011·International Journal of Cancer. Journal International Du Cancer·Dorien C RijkaartChris J L M Meijer
Feb 11, 2012·International Journal of Cancer. Journal International Du Cancer·Peng GuanGary M Clifford

❮ Previous
Next ❯

Citations

Jan 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M G DijkstraC J L M Meijer
Apr 17, 2015·Journal of Medical Virology·Sotirija DuvlisDijana Plaseska-Karanfilska
Oct 7, 2014·International Journal of Cancer. Journal International Du Cancer·Pierre-Marie TebeuPatrick Petignat
May 12, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Roosmarijn LuttmerChris J L M Meijer
May 26, 2009·Gynecologic Oncology·Philip E CastleWalter Kinney
Jul 28, 2016·Expert Review of Molecular Diagnostics·Roosmarijn LuttmerChris J L M Meijer
Nov 26, 2013·Journal of Lower Genital Tract Disease·Sarah Kay GoodrichJerome L Belinson
Sep 10, 2014·Frontiers in Oncology·Georges Herbein, Amit Kumar
Oct 13, 2020·Expert Review of Molecular Diagnostics·Mariano A MolinaKarolina M Andralojc
Jul 19, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Lynette Denny, Rose Anorlu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.